菟丝子水提液对大鼠的急性毒性及微核试验  

Acute Toxicity Test and Micronucleus Test of Water Extract from Cuscutae Semen in Rats

作　　者：夏卉芳[1,2] 李啸红[1] 

机构地区：[1]遵义医学院珠海校区,广东珠海519041 [2]贵州师范学院,贵阳550018

出　　处：《中国实验方剂学杂志》2014年第13期185-188,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省科学技术基金项目(黔科合J字LKZ[2010]38)

摘　　要：目的:探讨菟丝子水提液对SD大鼠的最大给药量的急性毒性与遗传毒性。方法:最大给药量法检测菟丝子水提液对SD大鼠的急性毒性。SD孕鼠骨髓微核试验及胚胎肝转移微核试验,设5组:菟丝子水提液高、中、低剂量组(40,20,10g·kg-1)及阴性对照组(NS,10 mL·kg-1),阳性对照组[环磷酰胺(CP)40 mg·kg-1],检测孕鼠骨髓嗜多染红细胞微核率及胚胎肝嗜多染红细胞微核率。结果:菟丝子水提液ig对SD大鼠的最大给药量为80 g·kg-1。菟丝子水提液40 g·kg-1可致孕鼠骨髓细胞、胚胎肝细胞嗜多染红细胞微核率升高,与阴性对照组对比,有显著性差异(P<0.01)。结论:菟丝子水提液对SD大鼠ig最大给药量为80 g·kg-1,属无毒级。菟丝子水提液ig剂量在40 g·kg-1时可诱发孕鼠及胚胎微核率增加,有潜在遗传毒性;而在20 g·kg-1以下剂量时无显著遗传毒性。Objective： To study the acute toxicity and inheritance toxicity of water extract of Cuscutae Semen in SD rats. Method： The acute toxicity of water extract of Cuscutae Semen in SD rats was detected by maximum tolerated dose method. Micronucleus test of pregnant mice bone narrow cells and fetal mice hepatocytes were carried out： 5 groups were used for bone narrow and fetal hepatic cell, include high, middle and low dosage of water extract of Cuscutae Semen （40, 20, 10 g·kg^- 1, respectively） , negative control group （ normal sodium, NS, 10 mL·kg^-1） and positive control group, the micronucleus rates of bone marrow and fetal hepatic cell were evaluated by mieronucleus tests. Result： The maximum dosage of administration of water extract Cuscutae Semen was 80 g ·kg^-1. Compared with negative control group, the micronucleus rates of bone marrow and fetal hepatic cell were all significantly increased （P 〈0.01 ） at a high dose of 40 g·kg^-1. Conclusion： The maximum tolerance dose of water extract of Cuscutae Semen （80 g ·kg^-1） in SD rats is belonged to the non-toxic substance. The high dosage of water extract of Cuscutae Semen （40 g ·kg^-1 ） has potential genetic toxicity on micronuclear rates of pregnant mice bone marrow cell and fetal mice hepatocytes, but middle and low dosage of it （20, 10 g ·kg^-1） have no genetic toxicity.

关 键 词：菟丝子 骨髓嗜多染红细胞 胚胎 微核 

分 类 号：R285.5[医药卫生—中药学]