降粘胶囊中丹参酮ⅡA和丹酚酸B的含量测定  

HPLC method for the determination salvianolic acid B and tanshinone ⅡA in Jiangnian capsule

作　　者：杨蝉[1] 杨玉琴[1] 张丽艳[1] 赵成元 赵勇 苟正雷 张强[1] 张前飞[1] 

机构地区：[1]贵阳中医学院,贵州贵阳550002 [2]贵州省遵义市人民医院563000

出　　处：《时珍国医国药》2014年第6期1354-1356,共3页Lishizhen Medicine and Materia Medica Research

基　　金：贵州省中药现代化科技产业研究开发专项([2010]5015号)

摘　　要：目的建立降粘胶囊中丹参酮ⅡA和丹酚酸B的含量测定方法。方法采用高效液相色谱法,ZORBX SB-C18色谱柱（150mm×4.6mm,5μm）,以甲醇∶水（75∶25）、甲醇-乙腈-甲酸-水（30∶10∶1∶59）为流动相;检测波长：270nm,284 nm;流速1.0ml·min-1;柱温：25℃。结果丹参酮ⅡA、丹酚酸B的浓度在0.064∽0.304μg（r=0.999 9）、0.56∽2.66μg（r=0.999 0）范围内线性关系良好,平均加样回收率为100.35%,（RSD为2.038%,）、99.77%（RSD为2.75%）。结论所建立的方法简单、灵敏、准确、重复性好。可用于降粘胶囊的质量控制。Objective To develop an HPLC method for the determination of salvianolic acid B and tanshinone Ⅱ A in Jiangnian capsule. Methods The sample was separated on ZORBX SB - ClS （ 150mm × 4.6mm, 5 μm） , with CH3 OH： HE O （ 75 ： 25 ） , CH3 OH - CH3 CN - methanoic acid - H20 （ 30： 10： 1 ： 59） solution by elution ; UV detection wavelength was at 270nm,284nm ; flow rate：l. 0mL/min; column temperature was at 25℃ Results The concentration of salvianolic acid B and tanshinone 1I A in Jiangnian capsule within 0.064- 0.304 μg （ r = 0.999 9 ） ,0.56 - 2.66 μg （ r = 0. 999 0 ） can have a good linear relationship, av- erage recovery is 100.35% （ RSD = 2.038% ） ,99.77% （ RSD = 2.75% ）. Conclusion it is a simple, sensitive and good repeat- ability method and can be used to control the quality of Jiangnian capsule.

关 键 词：降粘胶囊 高效液相色谱 丹参酮ⅡA 丹酚酸B 

分 类 号：R284.1[医药卫生—中药学]