随机扩增多态性DNA分析法在幽门螺杆菌鉴定中的应用  

Application of random amplified polymorphic DNA analysis in the identification of Helicobacter pylori strains

作　　者：胡素侠[1] 杨立新[2] 程龙强[1] 蔡霞[1] 张荣波[3] 

机构地区：[1]安徽省淮南市第一人民医院检验科,232001 [2]安徽省淮南市第一人民医院耳鼻喉科,232001 [3]安徽理工大学分子免疫实验室,安徽淮南232001

出　　处：《检验医学与临床》2014年第12期1623-1624,1627,共3页Laboratory Medicine and Clinic

基　　金：安徽省淮南市科技计划项目(2012A01402)

摘　　要：目的探讨随机扩增多态性DNA（RAPD）分析法在在幽门螺杆菌（HP）菌株鉴定中的应用价值。方法选取该院65例HP感染的慢性胃炎或消化性溃疡患者,获取胃窦、胃体部黏膜进行HP培养,提取及纯化HP DNA后进行PCR反应,并采用RAPD分析HP基因指纹图谱;以同一HP菌株验证RAPD可重复性;65例患者均进行6个月以上的HP治疗,治疗后4∽6周评估HP治愈情况;对复发病例再次进行RAPD分析,观察RAPD指纹图谱变化。结果 65株菌株PAPD指纹图谱各不相同,但同一菌株传4代后,原代株与传代株进行PCR扩增,RADP指纹图谱完全一致。6例HP治疗后复发,3例RAPD指纹图谱与治疗前基本一致;3例产生不同RAPD指纹图谱。结论 RAPD分析法适用于HP基因多态性鉴定,是临床研究HP感染的实用方法,可用于HP复发和再感染鉴定。Objective To investigate application value of random amplified polymorphic DNA analysis in the identifi-cation of HP .Methods 65 patients with peptic ulcer or chronic gastritis ,which confirm as HP infection ,get orget gastric body mucosa carried HP culture ,after HP DNA extraction and purification ,and analysis using HP RAPD genetic finger-printing ;strains of the same HP RAPD verify repeatability ,65 patients underwent six months or more HP eradication thera-py ,assess the situation Hp eradication 4-6 weeks after treatment ;recurrence again on RAPD analysis ,the changes of RAPD fingerprint were observed .Results 65 strains PAPD fingerprints are different ,but RADP fingerprints of the original genera-tion plants and extend the plant after 4 passages is same .Three cases is consistant in RAPD fingerprinting befor and after treatment ,3 cases have different RAPD fingerprints in six cases of relapse after HP eradication .Conclusion RAPD analysis of genetic polymorphisms identified for HP ,is a useful tool for clinical studies ,it can be used to identify HP relapse and rein-fection .

关 键 词：幽门螺杆菌 随机扩增多态性DNA 菌株 基因多态性 

分 类 号：R384.1[医药卫生—医学寄生虫学]