樟脑分子印迹整体柱的制备及性能评价  

Preparation and evaluation of camphor monolithic molecularly imprinted column

作　　者：孙蕾 鹿毅 杨涛 封顺[2] 成文虎[2] 曹艳红[2] 

机构地区：[1]新疆产品质量监督检验研究院,乌鲁木齐830004 [2]石油天然气精细化工教育部和自治区共建重点实验室,新疆大学化学化工学院,乌鲁木齐830046

出　　处：《分析试验室》2014年第7期791-795,共5页Chinese Journal of Analysis Laboratory

基　　金：自治区科技支疆项目(201191170)资助

摘　　要：利用原位聚合法制备了樟脑分子印迹整体柱作为液相色谱固定相,通过优化制备条件和色谱条件,得到了对模板分子有较高保留性能的整体柱。采用前沿色谱分析法测定柱容量,得到有效结合位点Bt=1.815×102mmol/g和解离常数Kd=9.064 mmol/mL。Van't Hoff方程得到的热力学参数焓值和熵值,说明了聚合物对樟脑的吸附为熵增过程。等温吸附模型证明在该聚合物表面只存在一种结合位点。Camphor monolithic molecularly imprinted polymers（monolithic MIPs） were prepared by in situ polymerization method as the liquid chromatography stationary phase.By optimizing the polymerization and chromatographic conditions,the monolithic MIPs showed high selectivity for the template.The binding capacity was evaluated by frontal analysis.The monolithic MIP had an effective number of binding sites Bt = 1.815 × 10^2mmol/g with a dissociation constant of Kd= 9.064 mmol/mL.Thermodynamic data ΔH and ΔS obtained by Van＇t Hoff plots revealed that the adsorption of camphor is an entropy production process.Isothermal adsorption analysis indicated that only one class of binding sites existed in this MIP.The experimental results testified that the prepared MIP had high selectivity and specificity to camphor in one real rosemary essential oil sample,and this means the MIPs can be used for direct analysis as a new method.

关 键 词：分子印迹聚合物 整体柱 原位合成法 樟脑 高效液相色谱 

分 类 号：O657.63[理学—分析化学]