HPLC-MS/MS同时检测5种甜菊糖苷类化合物  

Determination of five steviosides by HPLC-MS/MS

作　　者：顾莹[1] 盛永刚[1] 韩丽[1] 樊祥[1] 陈迪[1] 张润何[1] 邓晓军[1] 王敏[1] 

机构地区：[1]上海出入境检验检疫局,上海200135

出　　处：《分析试验室》2014年第7期796-798,共3页Chinese Journal of Analysis Laboratory

基　　金：质检公益行业科研专项(20111001904)资助

摘　　要：应用HPLC-MS/MS法同时检测了食品中5种甜菊糖苷类化合物：甜菊糖甙,瑞鲍迪甙A,瑞鲍迪甙C,杜克甙A和甜菊双糖甙的含量。样品用水超声提取,5种甜菊糖苷类化合物的定量限（LOQ）为1.7～3.4 mg/kg,样品在10,50,200 mg/kg添加水平的回收率为78%～107%,相对标准偏差（RSD）为4.9%～11%。方法可用于多种甜菊糖甙苷类的检测。A liquid chromatography tandem mass spectrometric method was developed for the determination of stevioside,rebaudioside A,rebaudioside C and steviolbioside in food.Samples were sequentially extracted by water,and then determined with HPLC-MS/MS.The limits of quantification of five compounds were 1.7 ～3.4 mg/kg.The recoveries at three spiked levels of 10,50 and 200 mg/kg were in the range of 78% ～ 107%,with RSDs（n = 6） of 4.9% ～ 11%.The proposed HPLC-MS/MS methods can be applied to the routine quality control of Stevia leaves and their commercial preparations.

关 键 词：甜菊糖甙 瑞鲍迪甙A 瑞鲍迪甙C 杜克甙A 甜菊双糖甙 液相色谱-串联质谱 

分 类 号：O657.63[理学—分析化学]