对二甲基氨基偶氮苯染料共振瑞利散射法测定红霉素  

Resonance Rayleigh scattering method for the determination of erythromycin with N,N-dimethyl-4-(phenyldiazenyl) benzene dyes

作　　者：陈莲惠[1] 简易[2] 冉利[1] 曹洪斌[1] 

机构地区：[1]川北医学院化学教研室,南充637000 [2]川北医学院物理教研室,南充637000

出　　处：《分析试验室》2014年第7期831-834,共4页Chinese Journal of Analysis Laboratory

基　　金：四川省教育厅重点项目(12ZA056);川北医学院重点培育项目(CBY11-A-ZP13)资助

摘　　要：在pH 4的HCl介质中,对二甲基氨基偶氮苯染料在50℃下可与红霉素作用形成稳定的离子缔合物。冷却至室温后在荧光分光光度计上同步扫描得到各溶液的共振瑞利散射光谱,发现单一的红霉素溶液的最大响应波长在334 nm附近,波峰和坡度变化均不明显;而与对二甲基氨基偶氮苯染料反应后最大响应波长增加到368 nm（甲基橙体系）和356 nm（甲基红体系）附近,在597 nm附近还有一肩峰,波峰、波谷分明;散射强度I也明显增强,且强度与红霉素质量浓度成正比,分别在0～50 mg/L或0～80 mg/L范围内符合Beer定律,线性回归方程为：IRRS=7.745×10^4ρ-15.17,r=0.9855（对甲基橙体系）,IRRS=1.139×10^5ρ-36.91,r=0.9913（对甲基红体系）。据此,建立了测定红霉素的共振瑞利散射新方法,检出限可达0.15μg/mL。方法应用于红霉素肠溶片中红霉素的质量分析,加标回收率在97.6%～103.9%之间。In pH4 hydrochloric acid medium,methyl orange or methyl red reacts with erythromycin in water solution to form complexes at 50 centigrade,which results in great enhancement of resonance Rayleigh scattering（RRS） and the new RRS spectra.The maximum RRS peaks are near 368 nm for methyl orange-erythromycin system and 356 nm for methyl red-erythromycin system,RRS intensities are proportional to the concentration of erythromycin.Beer＇s law is obeyed in the range of 0-50 mg/L（for methyl orange-erythromycin system） or0-80 mg/L（for methyl red-erythromycin system） of erythromycin.The linear equations are IRRS= 7.745 ×10^4ρ-15.17（r = 0.9855） and IRRS= 1.139 × 10^5ρ-36.91（r = 0.9913） independently.Based on these,a new RRS method for the determination of erythromycin was developed.The detect limits（3a） of these methods are found to be 0.15 μg/mL and 0.21 μg/mL separately.The method is reliable,repeatable,sensitive,and it has good selectivity.The proposed method has been successfully applied to the determination of erythromycin in its enteric-coated tablets with the recoveries between 97.6% and 103.9%.

关 键 词：甲基橙 甲基红 红霉素 共振瑞利散射法 

分 类 号：O657.3[理学—分析化学]