固相萃取-高效液相色谱法测定果蔬中异菌脲残留量  

Determination of iprodione residues in fruits and vegetables by solid phase extraction-high performance liquid chromatography

作　　者：郭藤[1] 李娜[1] 张洋[1] 李耀龙[1] 李珊 许秀丽[1] 

机构地区：[1]中国检验检疫科学研究院综合检测中心,北京100123 [2]山东鲁丰集团,潍坊262100

出　　处：《分析试验室》2014年第7期860-863,共4页Chinese Journal of Analysis Laboratory

摘　　要：建立了固相萃取-高效液相色谱法测定果蔬中异菌脲残留量的方法。样品用乙腈提取,C18固相萃取柱净化,甲醇-水（70：30,V/V）为流动相,经C18液相色谱柱分离,DAD紫外检测器（218 nm）检测。结果表明：异菌脲在0.1～2.0 mg/L范围内线性关系良好（R2=0.9998）,方法定量限（以S/N=10计）为0.05 mg/kg,在0.05,0.1,0.5 mg/kg添加水平下的加标回收率范围为81.3%～98.3%,相对标准偏差（n=6）为3.9%～8.9%。方法适用于大多数水果、蔬菜中异菌脲残留量的测定。A method for the determination of iprodione residues in fruits and vegetables by high performance liquid chromatography（HPLC） was developed.The samples were extracted with acetonitrile,cleaned up with C18-solid phase extraction（SPE）,then separated on a C18 chromatographic column with acetonitrile and water（70：30,V/V） mixed solution as the mobile phase and were detected by an ultraviolet detector at 218 nm.The linear range was 0.1 ～ 2.0 mg/L,the correlation coefficient was 0.9998 and the limit of quantification（S/N =10） was 0.05 mg/kg.The recoveries were 81.3% ～ 98.3% with the relative standard deviations（n = 6） of4.4% ～ 8.9% at the three different spiked levels of 0.05,0.1 and 0.5 mg/kg.The method is simple,accurate,and reproducible,and consums less sample and organic solvents.It can be applied to the determination of iprodione residues in fruits and vegetables.

关 键 词：异菌脲 高效液相色谱法 固相萃取(SPE) 基质分散固相萃取(QuEChERS) 

分 类 号：O657.7[理学—分析化学]