银杏叶提取物对免疫抑制小鼠免疫功能的影响  

EFFECT OF GINKGO BILOBA EXTRACT ON IMMUNE FUNCTION IN IMMUNOSUPPRESSED MICE

作　　者：徐芳[1] 贺兰 毛宇[1] 徐小娟[1] 贺建华[3] 

机构地区：[1]湖南农业大学食品科技学院,长沙410128 [2]益阳市赫山区沧水铺人民医院,益阳413064 [3]湖南农业大学动物科技学院,长沙410128

出　　处：《营养学报》2014年第3期268-272,共5页Acta Nutrimenta Sinica

基　　金：科技部国际科技合作专项基金(No.2011DFA30490)

摘　　要：目的探讨银杏叶提取物(Ginkgo biloba extract,EGB)对环磷酰胺(cyclophosphanmide,CTX)免疫抑制小鼠的免疫调节作用。方法健康昆明小鼠150只,随机分为对照组(Control)、CTX、EGB低、中、高剂量(EGB4、10、20 mg/kg bw)5组。实验前CTX和EGB各组腹腔注射CTX 100 mg/kg bw 3 d,之后EGB各组对应灌胃EGB 4、10、20 mg/kg bw,对照组和CTX组灌胃等容积生理盐水,1/d,连续15 d后测定免疫器官指数、廓清指数、血清溶血素、脾淋巴细胞增殖和细胞因子等免疫指标。结果与对照组比较,CTX组脾脏指数、胸腺指数、校正廓清指数、血清溶血素、脾淋巴细胞增殖能力和血清IL-6水平等均显著降低(P<0.01或P<0.05)。与CTX组比较,EGB-M、EGB-H剂量组脾脏指数显著提高(P<0.01,P<0.05),EGB-L组的胸腺指数无显著差异,EGB-M、EGB-H组胸腺指数显著增加(P<0.05,P<0.01),且呈剂量效应。EGB-H组的校正廓清指数和血清溶血素水平与CTX组相比均有差异显著性(P<0.05),且呈剂量效应。与CTX组比较,EGB-M、EGB-H组脾淋巴细胞的增殖能力和小鼠血清IL-6水平显著增加(P<0.01或P<0.05),且淋巴细胞增殖能力呈明显剂量效应,血清IFN-γ水平无显著差异。结论 EGB可以提高免疫抑制小鼠的非特异性免疫、体液免疫和细胞免疫功能。Objective To explore the effect of Ginkgo biloba extract（EGB） on immune function in immunosuppressive mice. Methods One hundred and fifty healthy Kunming mice were randomly divided into normal control group（control, given normal saline）, immunosuppressed model group（CTX） and low, medium and high doses treatment groups（EGB-L, EGB-M, EGB-H ig 4,10,20 mg / kg bw）. Except control group, the other groups were treated with intraperitoneal injection of CTX（100mg/kg bw） for 3d; and each group was gavaged once daily for 15d. Then, immune organ index, clearance index, serum hemolysin, spleen lymphocyte proliferation, cytokines and other immune parameters were measured. Results Compared to the control group, the changes of spleen index, thymus index, calibration clearance index, serum hemolysin, spleen lymphocyte proliferation and serum IL-6 levels decreased significantly（P〈0.01 or P〈0.05） in CTX group. Compared to the CTX group, spleen index significantly increased（P〈0.01, P〈0.05） in EGB-M,EGB-H group. Thymus index had no significant difference between EGB-L group and CTX group and significantly increased（P〈0.05, P〈0.01） in EGB-M, EGB-H groups in a dose dependent manner. Compared to the CTX group, the corrected dearance index and serum hemolysin level had a significant difference（P〈0.05） and lymphocyte proliferation and serum IL-6 levels were significantly increased（P〈0.01 or P〈0.05） in EGB-M and EGB-H groups and serum IFN-γ level had no significant difference. Conclusion EGB can increase non-specific humoral and cellular immune functions in immunosuppressed mice.

关 键 词：银杏叶提取物 免疫抑制小鼠 免疫功能 

分 类 号：R285.5[医药卫生—中药学]