黄淮麦区小麦春化基因组成的多态性分布研究  

The polymorphism distribution of the vernalization genes in wheat cultivars in Huang-huai wheat region

作　　者：郭总总 王翔[1] 卫丽[1] 白瑞英[1] 曹云[1] 郭创[1] 尹钧[1] 

机构地区：[1]河南农业大学国家小麦工程技术中心,河南郑州450002

出　　处：《河南农业大学学报》2014年第3期255-262,共8页Journal of Henan Agricultural University

基　　金：国家科技支撑计划项目(2011BAD16B07;2013BAD04B01)

摘　　要：为了明确黄淮麦区春化基因显隐性组成在小麦品种中的分布情况,揭示品种的发育特性.以Vrn-A1,VrnB1,Vrn-D1和Vrn-B3以及Vrn-D1启动子区单核苷酸突变引物为STS分子标记,对黄淮麦区的91个品种进行了分子标记检测.结果显示,所有供试材料在Vrn-A1和Vrn-B3位点均检测为隐性.在Vrn-B1位点,有6个品种检测为显性(6.6%),其余均为隐性.在Vrn-D1位点,黄淮麦区的品种中有38份材料为显性基因(41.8%),53份为隐性基因(58.2%),在38份显性材料中,有18份材料为Vrn-D1a类型(47.4%),有20份材料为Vrn-D1b类型(52.6%).研究认为,Vrn-D1a和Vrn-D1b可以作为黄淮麦区小麦品种的弱春性和半冬性的有效分子标记.In order to identify the distribution characteristics of the allelic composition of the vernaliza- tion genes and its relationship with growth habit of wheat （Triticum aestivum L. ） cuhivars in Huang- huai wheat region, the allelic composition of the vernalization gene Vrn-A1, Vrn-B1, Vrn-D1, Vrn-B3 and single nucleotide mutation primers in the loci of Vrn-D1 promoter region of 91 wheat cultivars were analyzed using the sequence-specific PCR. The results showed that no dominant allele of Vrn-A1 or Vrn-B3 was found in the wheat cuhivars in this study. 6 materials （6.6%） were dominant in the Vrn- B1 locus. In the loci of Vrn-D1, 38 materials （41.8%） in Huang-huai region had the dominant gene and 53 （58.2%） for the recessive gene in the Vrnl locus. There were 18 germplasms for the Vrn-Dla type （47.4%） and 20 Vrn-Dlb （52.6%） type in the 38 dominant cultivars. The results showed that Vrn-Dla and Vrn-Dlb were an effective molecular marker for weak spring and facultative wheat in huang-huai region.

关 键 词：普通小麦 春化基因 STS分子标记 Vrn-D1 

分 类 号：S512.1[农业科学—作物学]