基因芯片对哮喘小鼠趋化因子及其受体差异表达的筛选与分析  

Screening and analysis of chemokines and their receptors expression in murine asthma model using gene microarray

作　　者：岳欢[1,2] 李燕[3] 高婧[1,2] 江涛[1,2] 黄俊琼[1,2] 

机构地区：[1]遵义医学院附属医院检验科 [2]遵义医学院检验系,贵州遵义563099 [3]四川省仁寿县疾病预防控制中心,四川眉山620500

出　　处：《遵义医学院学报》2014年第3期286-289,共4页Journal of Zunyi Medical University

基　　金：国家自然科学基金资助项目(NO:81260266);贵州省优秀科技教育人才省长专项基金项目(NO:黔省专合字200951)

摘　　要：目的用SABioscience基因芯片检测小鼠哮喘模型中84个趋化因子及其受体基因,并初步筛选分析其与支气管哮喘的相关性。方法用OVA构建哮喘小鼠模型,于末次激发后取右肺组织进行HE染色,并提取左肺组织总RNA,用SABioscience芯片检测84个趋化因子及其受体基因的表达水平。结果成功构建哮喘小鼠模型,哮喘组与对照组相比,表达上调的趋化因子21个,趋化因子受体13个;表达下调的趋化因子4个。结论 SABioscience芯片可有效地筛选出哮喘小鼠差异表达的趋化因子及其受体,对进一步深入探讨哮喘的发病机制及预防具有重要意义。Objective To test the expression of 84 chemokines and chemokines receptors genes in murine asthma model using SABioscience gene microarray and preliminarily screen and analyze their relationship to asthma.Methods Murine asthma model was established through i.p.injection of OVA and aerosol challenge.Within24 hours after-last stimulating,the right lung tissue of mice was stained by HE staining.Total RNA was extracted from left lung tissue and the expression level of 84 chemokines and chemokine receptors were analyzed by SABioscience gene microarray.Results Murine asthma models was successfully established.21 chemokines and16 chemokine receptors are over two fold up-regulated and 4 chemokines are over two fold down-regulated in asthma group,compared with those in control group.Conclusion Differently expressed chemokines and chemokine receptors of asthma mice could be effectively filtered out by SABioscience microarray,which provided the foundation for further exploration of the pathogenesis mechanism and prevention of asthma.

关 键 词：支气管哮喘 趋化因子 受体 基因芯片 

分 类 号：R392.8[医药卫生—免疫学]