酶联免疫转印技术(EITB)在检测人类脑囊虫病患者血清IgG和IgG_4抗体中的应用  

ENZYME LINKED IMMUNOELECTROTRANSFER BLOT ASSAY (EITB)FOR DETECTING IGG AND IGG 4 ANTIBODY IN SERUM OF HUMAN NEUROCYSTICERCOSIS

作　　者：吴静[1] 李雅杰[1] 刘平[1] 王慧[1] 

机构地区：[1]哈尔滨医科大学寄生虫学教研室,150086

出　　处：《寄生虫与医学昆虫学报》2001年第1期13-18,共6页Acta Parasitologica et Medica Entomologica Sinica

摘　　要：本试验对 4组脑囊虫病患者血清中的总 Ig G和 Ig G4亚类抗体水平进行研究。第 1组由未经治疗的 ,通过寄生虫学及临床表现确诊的感染者组成。第 2 ,3,4组由经过 1～ 3,4～ 6和 7～ 9疗程治疗的治疗后患者组成(每一疗程为 10天 ,吡喹酮治疗 ,剂量为 30 m g/kg.d)。用植物亲和层析纯化抗原 ,通过 EITB,检测 4组共 2 4 1份血清样品中的 Ig G和 Ig G4含量。对照组为采用健康个体的 36份血清。另外还检测了 2 7份肝包虫和肝吸虫患者血清。通过与对照组比较 ,4组中的总 Ig G和 Ig G4抗体水平分别为 96 .3%和 97.5% ,93.3%和 78.6 % ,88.0 %和 38.0 % ,86 .1%和 13.9%。4组中的 Ig G水平无显著差异 ( P>0 .0 1)。相对的 ,治疗后患者的 Ig G4抗体水平低于治疗组。对有症状的治疗后患者 ,检测其特异的抗体水平发现 77.1%的患者 Ig G4水平治疗前后有显著差异 ( P<0 .0 0 1) ,而只有 2 1.8%的患者没有显著差异。对于这四组而言 ,被 Ig G识别的抗原没有一种是独特的。许多患者的 Ig G最常识别 GP4 2和 GP2 4这两条带 ,但在最后两组中 ,Ig G4能特异识别 18k Da和13k Da低分子量抗原。36份阴性对照均为阴性。2 7份其他寄生虫感染中有 2例总 Ig G阳性 ,而 Ig G4均为阴性。这些观察结果表明 ,IgTotal IgG and IgG 4 subclass antibody were investigated in serum of neurocysticercosis patients who were divided into four groups.The first group consisted of parasitological and clinically confirmed patients before treatment.The second,third and fourth groups comprised treated patients after 1~3,4~6 and 7~9 therapeutic courses respectively(Each therapeutic course includes 10~14 days) *.241 serum samples from these four groups were detected with EITB using lentil lectin affinity purified antigens for total IgG and IgG 4.36 sera from healthy individuals were used as negative control.We also detected 27 sera from patients of echinococcosis and clonorchiosis by the same method.Compared to negative control the total IgG and IgG 4 subclass antibody levels in four different groups were respectively 96 3% and 97 5% for the lst group;93 3% and 78 6% for the 2ed;88 0% and 38 0% for the 3rd;86 1% and 13 9% for the 4th.There was no significant difference for the total IgG among these four groups( P >0 01).In contrast,the levels of IgG 4 antibodies in the post treatment patients were lower than that of the before treatment patients.The positive rate of IgG 4 antibody in symptomatic post treatment patients is 77 0% but in asymptomatic post treatment patients is only 21 3%( P <0 001).None of the antigens recognized by IgG was unique to the four groups.GP42 and GP24 were the most common bands recognized by many patients for IgG,but in the latter two groups,IgG 4 distinctively recognized low molecular weight antigen of 18kD and 13kD.36 sera from healthy individuals were all negative.The positive rate of total IgG antibody in 27 sera from heterologous infections is 7 5%,in contrast,IgG 4 antibody of these sera were all negative.These observation confirmed that IgG 4 is an important diagnostic parameter for cure of human neurocysticercosis.

关 键 词：脑囊虫病 总IgG IgG4亚类 EITB 酶联免疫转印技术 检测 

分 类 号：R446.62[医药卫生—诊断学]