心室成纤维细胞条件培养液促进成纤维细胞胶原合成和增殖  

Role of conditioned growth medium for ventricular fibroblasts in promoting fibroblast collagen synthesis and proliferation

作　　者：龚素珍[1] 刘培庆[1] 鲁伟[1] 王庭槐[1] 符史干[1] 潘敬运[1] 

机构地区：[1]中山医科大学生理学教研室,广州510089

出　　处：《生理学报》2001年第1期18-22,共5页Acta Physiologica Sinica

摘　　要：采用心室成纤维细胞条件培养液培养心室成纤维细胞 ,通过测定 [3H] 脯氨酸 ([3H] proline)的掺入率来了解心室成纤维细胞总胶原合成速率 ,通过测定 [3H] 胸腺嘧啶核苷 ([3H] TdR)的掺入率以及c fos基因的表达丰度来了解心室成纤维细胞的增殖速率。结果显示 :心室成纤维细胞条件培养液 (FCGM)能明显增加细胞自身的[3H] proline的掺入率和 [3H] TdR的掺入率 ,并具有剂量依赖性 ;FCGM也能促进细胞自身c fos基因的表达 ,刺激后 1h达高峰。ETA 受体拮抗剂BQ12 3能部分阻断FCGM增加成纤维细胞胶原合成和增殖作用 ,而AT1受体拮抗剂CV11974和α 肾上腺素受体拮抗剂regitin无此效果。结果提示 :心室成纤维细胞具有自分泌功能 ,能分泌内皮素等生物活性物质 。Ventricular fibroblasts were cultured using conditioned growth medium for ventricular fibroblasts (FCGM). The rate of the total collagen synthesis of ventricular fibroblasts was measured by assaying the incorporation rate of [ 3H] proline, whereas the proliferation of ventricular fibroblasts was assessed by determining the incorporation rate of [ 3H] TdR and the expression of c fos genes. FCGM significantly increased the [ 3H] proline incorporation rate and [ 3H] TdR incorporation rate of fibroblasts in a dose dependent manner. Furthermore, FCGM promoted the c fos gene expression of fibroblasts, which attained its maximum in 1 h. BQ 123 , an ET A receptor antagonist, partially blocked the above effects of FCGM, but AT 1 receptor antagonist CV11974 and α adrenergic receptor antagoist regitin did not. It is suggested that the ventricular fibroblast has an autorine function in promotion of collagen synthesis and proliferation of fibroblasts by secreting endothelin and other bioactive substances.

关 键 词：心室成纤维细胞 [^3H]-脯氨酸 [^3H]-胸腺嘧啶核苷 C-FOSMRNA 心脏 

分 类 号：R331.1[医药卫生—人体生理学]