猕猴精浆纤溶酶原激活因子的来源及在精子获能中的作用  

Source of plasminogen activator in rhesus monkey semen and its possible role in sperm capacitation

作　　者：郑萍[1] 邹如金[1] 刘以训[2] 

机构地区：[1]中国科学院昆明动物研究所生殖生物学实验室,昆明650223 [2]中国科学院动物研究所生殖生物学国家重点实验室,北京100080

出　　处：《生理学报》2001年第1期45-50,共6页Acta Physiologica Sinica

基　　金：ThisresearchwassupportedbytheNationalNaturalScienceFoundationofChina (No 39810 36 8) ;theMinistryofScienceandTechnologyofChina

摘　　要：我们的前期工作表明 ,不育症人精液中纤溶酶原激活因子 (plasminogenactivator;PA)活性明显升高 ;给成年男子和猕猴注射长效睾酮诱发无精过程中 ,精液PA含量也伴随上升。为进一步查明PA的来源和对精子的作用 ,用原位杂交检测组织型PA (tPA) ,尿激酶型PA (uPA)及PA抑制因子 1(PAI 1)的mRNAs在成年健康猕猴附睾、前列腺和精囊中的表达。体外培养猕猴精子 ,培液中加入uPA、tPA及其底物纤溶酶原 (plasminogen) ,测试PA对精子活力、顶体反应及激活卵子的影响。结果表明 ,猕猴附睾、前列腺和精囊均表达tPA、uPA和PAI 1mR NAs。加入uPA能维持精子的活力 ,使精子产生超激活运动 ,诱导顶体反应的发生 ,并使精子获得激活卵子的能力。这说明猕猴精浆PA除来源于睾丸外 ,可能主要来源于附睾及附性腺 ;在体外 ,uPA ,而不是tPA 。Previous studies have demonstrated that plasminogen actiator (PA) activity is significantly high in semen of infertile men, which is also high in semen when azoospermia or oligozoospermia is induced by injection of testosterone enanthate (TE) into healthy adult men or rhesus monkeys. To further clarify the source and possible role of PA in semen, the present study was undertaken to examine: (1) whether the mRNAs for tissue type PA (tPA), urokinase type PA (uPA), and PA inhibitor 1 (PAI 1) are expressed in epididymis, seminal vesicle and prostate gland of rhesus monkeys; and (2) whether PA has some effect on in vitro sperm capacitation as judged by the potential of sperm motility, acrosome reaction (AR) and in vitro fertilization. Our results showed that (1) mRNAs for PA and PAI 1 were expressed in epididymis, seminal vesicle and prostate gland, and (2) uPA, but not tPA, improved sperm mobility, induced AR and enhanced sperm capacity to fertilize mature eggs. Thus, it is concluded that PA activity in semen comes not only from testis and epididymis, but also from seminal vesicle and prostate gland; and that uPA, but not tPA, may play a role in sperm capacitation.

关 键 词：猕猴 纤溶酶原激活因子 精子获能 

分 类 号：R341[医药卫生—基础医学]