蟾蜍和小鼠延髓神经元Fos蛋白表达比较  

Comparison of Neuronal Fos Expressions of Medulla Oblongata in Toad and Mouse

作　　者：李颖[1] 艾洪滨[1] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014

出　　处：《济南大学学报（自然科学版）》2014年第2期142-146,共5页Journal of University of Jinan(Science and Technology)

基　　金：国家自然科学基金(31071920);山东省自然科学基金(ZR2011CM029)

摘　　要：将蟾蜍与小鼠分别分为实验组与对照组。实验组给予足跖皮肤针刺刺激,检测二者延髓平髓纹水平Ⅳ脑室周围神经元Fos蛋白的阳性表达情况。结果表明:蟾蜍实验组和对照组Fos阳性神经元数量均很多,组间差异无统计学意义;小鼠实验组Fos阳性神经元显著多于对照组(p<0.01);与蟾蜍比较,小鼠实验组和对照组Fos阳性神经元数量均显著少于蟾蜍(p<0.05,p<0.01)。提示蟾蜍和小鼠Ⅳ脑室周围神经元对足跖部皮肤的针刺刺激的敏感度不同。The comparison of the Fos expressions of medulla neurons in toad and mouse were made. Toads and mice were separately divided into two groups ： experimental group and control group. Gave the acupuncture stimulation to the skin of posterior limb vola pedis of the experimental animals,the control group animals were not given stimulation. The amount of Fos positive neurons around the IV ventricle fiat stria medullaris level in both of animals were determined by using immunohistochemical method. For toad, both the experimental group and the control group, there are a lot of Fos positive neurons in medulla, the differences between the two groups have no statistically significance. For mouse, compared with toads, both the experimental group and the control group, the amount of Fos positive neurons were significantly lower than toads （p 〈0. 05, p 〈 0.01 ） ; the amount of Fos positive neurons in the experimental group was significantly higher than that of the control group （p 〈 0. 01 ）. These results indicate that the neurons around the 1V ventricle in toad＇ s medulla are not sensitive to the acupuncture stimulation of the skin of posterior limb vola pedis and the mice are sensitive. The higher mammals may be due to the encephalization, the number of neurons in medulla in response to noxious stimulation of the skin is instead fewer than that of the lower vertebrates.

关 键 词：蟾蜍 小鼠 延髓 神经元 FOS蛋白 针刺刺激 

分 类 号：Q954.52[生物学—动物学]