光接枝技术构筑仿细胞外层膜两性离子表面  

Preparation of biomimetic zwitterionic surface by photografting method

作　　者：史素青[1] 赵洋[1] 刘景维[1] 张琴[1] 宫永宽[1] 

机构地区：[1]西北大学合成与天然功能分子化学教育部重点实验室/化学与材料科学学院,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2014年第3期416-422,共7页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(21004047;J1210057;J1103311);陕西省自然科学基础研究计划基金资助项目(2010JQ2004);教育部博士点新教师基金资助项目(20106101120005);陕西省教育厅专项科研计划基金资助项目(09JK767)

摘　　要：采用硅烷化反应将叔胺氢给体偶联到羟基化盖玻片表面,在含二苯甲酮（BP）光敏剂的2-甲基丙烯酰乙氧基磷酰胆碱（MPC）单体溶液中进行光接枝聚合.用X射线光电子能谱（XPS）对改性表面的化学结构进行表征,接触角对改性表面亲/疏水性能进行测定,蛋白质吸附和血小板黏附评价其抗生物污染性能.结果发现,与未改性表面相比,经含叔胺的硅烷化试剂处理后,基材表面形成了一层胺基化有机无机杂化过渡层;光接枝PMPC后表面于400.2 eV处和131.6 eV处分别出现了N峰和P峰,相对含量分别为4.47％和4.80％,且表面Si2p相对含量显著降低,同时,光接枝各步反应表面接触角变化可间接表明材料表面成功接枝PMPC聚合物.所得两性离子表面蛋白质吸附量降低了91.3％～92.4％,基本不发生血小板黏附.硅烷偶联剂的应用不仅能够提供表面接枝反应所需的自由基源,而且还可增强两性离子聚合物刷涂层与基材表面的黏结强度,对进一步研究涂层稳定性与血液相容性之间的关系奠定了基础.The tertiary amine hydrogen donor is immobilized to the hydroxylated substrate surface by silaniza- tion of N, N-dimethyl-3-aminopropyl trimethoxysilane （DATS） , and subsequently photoinduees the grafting polymerization of zwitterionic monomer MPC in combination with benzophenone （BP） in solution. X-ray pho- toelectron spectroscopy （XPS） is used to monitor the chemical changes on the glass surface. Dynamic and static water contact angles are used to study the hydrophiliphic/hydrophobie properties. Protein adsorption and blood platelet experiments are used to evaluate the antifouling properties. The result shows that a transitional organic-inorganic thin hybrid coating rich in tertiary amine groups is formed on the hydroxylated substrate sur- face. After photografting of MPC, the XPS signals for nitrogen and phosphorus element appears at 400.2 eV and 131.6 eV, respectively, and the content of Si on the surface evidently decreases simultaneously. Moreo- ver, the change of water contact angle for substrate surface before and after photografting can also indicate the successful fabrication of zwitterionic polymer brushes. The absorption of proteins on the obtained zwitterionic surface greatly reduces by 91.3% -92. 4% and almost no platelets adhesion oecurs. The silane agent contai- ning tertiary amine functional groups can not only provide the radical source for photografting polymerization but also enhance the adhesion between polymer coatings and the substrate surface, which is beneficial to the further study the relationship between stability of the coatings and the resulting blood compatibility.

关 键 词：光接枝 两性离子 硅烷偶联剂 蛋白质吸附 血小板黏附 

分 类 号：O631[理学—高分子化学]