山东省归国劳务人员中HIV-1感染毒株的序列特征和亚型分析  

Analysis of HIV 1 Sequence and Subtype in Returned Laborers Worked Abroad in Shandong Province

作　　者：苏生利[1] 邢辉[2] 刘传新[1] 潘品良[2] 黄涛[1] 傅继华[1] 赵世立[1] 邵一鸣 

机构地区：[1]山东省卫生防疫站,山东济南250014 [2]卫生部艾滋病预防与控制中心,北京100050

出　　处：《中国病毒学》2001年第1期34-39,共6页Virologica Sinica

基　　金：山东省卫生厅基金资助! ( 98年第 10 70 10 2号 )

摘　　要：为了解自 1992年以来 ,山东省归国劳务人员HIV 1感染毒株的分子流行病学特征。我们采集了 11份确认为HIV 1感染者的全血分离单核细胞 (PBMC) ,提取前病毒DNA ,用套式PCR扩增HIV 1膜蛋白 (env)基因 ,并对其envC2 V3区进行序列测定和分析。结果表明 :10名归国劳工人员及 1名归国劳工配偶的样本经序列测定和基因分析发现了HIV 1的 4种基因亚型 :A、B、C、E亚型。其中C亚型 7名 ,B亚型 2名 ,A和E亚型各 1名。由此可见 ;山东省归国劳务人员中HIV 1毒株的感染情况较复杂 ,通过计算发现这些毒株间的基因离散率相差较大。提示应加强归国劳务人员的教育。To understand the molecular epidemiological characteristic of HIV 1 virus strain in laborers worked abroad in Shandong province since 1992, 11 specimens of peripheral blood of identified HIV infected cases were sampled and PBMC was separated by Ficoll density gradient centrifuge. The previrus DNA was extracted and the membrane protein gene env DNA was multiplied by nested PCR, and then the sequence of env C2 V3 region was measured and analyzed. The results indicated that 10 laborers worked abroad and 1 female spouse of a laborer worked abroad were found catching HIV 1 A , B , C, E subtype by gene sequencing and analyzing, with subtype C 7, subtype B 2, subtype A and E 1 respectively. Thus it can be seen that the infection of HIV virus strain in the laborers worked abroad was complex. The gene variation rate of these virus strains was greatly different. It is suggested that we strengthen the detection and control of the laborers worked abroad to prevent the spread of the virus strains from other countries.

关 键 词：HIV-1 归国劳工 序列测定 亚型 系统树 人体免疫缺陷病毒 

分 类 号：R181.3[医药卫生—流行病学]