“非可培养细菌”培养策略研究进展  

The Research Progress in Cultivation Strategies of Uncultivated Bacteria

作　　者：邱玉兰[1] 井晓欢 杨季芳[1] 陈吉刚[1] 

机构地区：[1]浙江万里学院,浙江宁波315100

出　　处：《浙江万里学院学报》2014年第3期78-88,共11页Journal of Zhejiang Wanli University

基　　金：浙江省大学生科技创新项目"海洋微生物高通量分离装置研制及其评价"(2013R419006)

摘　　要：近年来,研究者利用培养策略增加了环境样品中可培养细菌的数量,但培养效率仍然是研究者当前所面临的最大挑战。环境样品中存在两类"不可培养"的细菌类群是影响培养效率的主要原因。一类是:该类群中目前尚无培养代表,其合适的培养条件尚没确定。另一类群是:该类群的细胞处于非分裂状态(也称不可培养的休眠或活细胞),需要移除或添加某些因子才能使其重新开始生长。文章综述了培养"非可培养细菌"的一系列策略以及影响培养策略的一些潜在机制,并强调:了解阻碍培养的因素是确定最佳培养策略的首要前提。Many strategies have been used to increase the number of bacterial cells that can be grown from environmental samples, but the cultivation efficiency remains a challenge for researchers. The difficulty of cultivating a fraction of bacteria in environmental samples can be classified into two non- exclusive categories. Bacterial taxa with no cultivated representatives for which appropriate laboratory conditions necessary for growth are yet to be identified. The other class is cells in a non-dividing state （also known as dormant or viable but not cuhurable ceils） that require the removal or addition of certain factors to re-initiate growth. A number of strategies, from simple to high throughput techniques, are reviewed that have been used to increase the cultivation efficiency of environmental samples. Some of the underlying mechanisms that contribute to the success of these cultivation strategies are described. Overall this review emphasizes the need of researchers to first understand the factors that are hindering cultivation to identify the best strategies to improve the cultivation efficiency.

关 键 词：非分裂细胞 培养效率 生长因子 

分 类 号：Q93[生物学—微生物学]