鮻鱼幼鱼肝脏和肌肉氧化应激指标对溶解氧含量变动的响应  

Response of Oxidative Stress Indicators in Liver and Muscle of Mullet Liza haematocheilato Variation in Dissolved Oxygen Levels

作　　者：刘旭佳[1] 黄国强[1,2] 李洁[2] 唐夏[2] 

机构地区：[1]广西海洋研究所海洋生物技术重点实验室,广西北海536000 [2]中国海洋大学水产学院,山东青岛266003

出　　处：《水产科学》2014年第6期344-349,共6页Fisheries Science

基　　金：山东省自然科学基金资助项目(ZR2009DM020);广西壮族自治区自然科学基金资助项目(2011GXNSFA018116)

摘　　要：研究了4种溶氧变动模式条件下,鮻鱼幼鱼肝脏和肌肉氧化应激指标的变化。试验结果表明,在"近饱和稳定"模式下,鱼体肝脏中氧化应激指标含量的变动比较一致,具有一定的变动节律,且肌肉中的大部分氧化应激指标高于肝脏。与"近饱和稳定"模式相比,"近饱和→最低溶氧"模式下肝脏大部分氧化应激指标显著升高,而肌肉中相应指标则明显下降。"近饱和→超饱和→近饱和→最低"模式肝脏的主要氧化应激指标变动的时间节律与"近饱和稳定"模式明显不同,并在次日溶解氧含量下降至最低值1.0mg/L时,抗氧化指标的含量有所回升。"超饱和→近饱和→近饱和恢复24h"模式下,肌肉中所有氧化应激指标与"近饱和稳定"模式相比均明显下降,并且变动节律与溶解氧含量的变动模式一致。在经历超饱和溶氧暴露后恢复24h处理后,肝脏的抗氧化指标,只有总抗氧化能力最高值出现在溶氧超饱和期间,超氧化物歧化酶和抗超氧阴离子活力的最高值出现在溶氧降低至近饱和水平后的恢复阶段,肌肉中的抗氧化指标最高值则均出现在恢复阶段。溶解氧含量变动环境中,鮻鱼的氧化应激指标与溶氧稳定的环境中相比在数值和变动节律上均出现变化,并表现出在溶解氧含量下降时储备抗氧化物质的现象。The changes in oxidative stress indicators were studied in liver and muscle of the juvenile mullet Liza haematocheila under four patterns of dissolved oxygen levels .The results showed that the variation in oxidative stress indicators was consistent and rhythmical in liver ,lower than in muscle in the pattern nearly constantly saturated with dissolved oxygen .Most of the oxidative stress indicators were found to be increased significantly in liver in the pattern from nearly saturated to the minimum value of dissolved oxy‐gen ,but the opposite case in the muscle .There was significantly difference in rhythm of the oxidative stress indicators in liver under the pattern from nearly saturated ,super‐saturated ,and nearly saturated to minimum value of dissolved oxygen compared with that in the pattern of saturated dissolved oxygen .How‐ever ,the level of the oxidative stress indicators was found to be increased on the next day when the content of the dissolved oxygen was reduced to 1 .0 mg/L .In the pattern from over‐saturation ,nearly saturation to recovery from nearly saturation ,the content of oxidative stress indicators in muscle was declined sharply compared to the pattern of nearly saturated dissolved oxygen ,with the consistent change in rhythm with the content of dissolved oxygen .The maximal value of T‐AOC was observed in the supersaturated period , and the maximal SOD and T‐SOD activities in liver were found in the 24 h recovery stage after the nearly saturated period .In the muscle ,however ,the maximum value of all the oxidative stress indicators was ob‐served in the recovery stage .The findings indicated that the oxidative stress indicators in different pattern showed different dissolved oxygen contents and rhythm compared to the normal dissolved oxygen condi‐tion .T he mullet had positive response to the variation in dissolved oxygen patterns and maintained antiox‐idant substance during the lower dissolved oxygen level period .

关 键 词：鮻鱼 溶氧 缺氧 氧化应激 抗氧化 

分 类 号：S965.234[农业科学—水产养殖]