流式细胞术分析强声波对植物细胞周期的影响  

ANALYSIS OF THE EFFECT OF STRONG SOUND WAVE ON PLANT CELLS CYCLES USING FLOW CYTOMETRY

作　　者：李涛[1] 侯月霞[2] 蔡国友[3] 沈子威[4] 席葆树[3] 陶祖莱[1] 

机构地区：[1]中国科学院力学研究所国家微重力实验室,北京100080 [2]中国农业大学应用化学系,北京100094 [3]清华大学工程力学系,北京100084 [4]清华大学生物科学与技术系,北京100084

出　　处：《生物物理学报》2001年第1期195-198,共4页Acta Biophysica Sinica

摘　　要：应用流式细胞术分析烟草细胞在交变应力作用下细胞周期的变化。用特制的强声波发生装置产生频率和强度可调的交变应力场 ,研究不同频率和强度的交变应力作用后烟草细胞周期的变化。实验结果表明 ,在交变应力作用下直接影响细胞或细胞分裂的同步化 ,促进S期的DNA合成 ,有助于细胞有丝分裂。声波频率在400Hz至800Hz,强度在90dB到110dB内 ,随频率和强度的增加 ,交变应力使S期细胞明显增加 ,但频率或强度过大 ,反而使S期细胞大大减少。Flow cytometry was used to measure cell cycle parameters in tobacco cell suspensions acted by alternative stress. The field of alternative stress was generated through a strong sound filed system. It was found that the effects of stress on cells depended greatly on the intensity and frequency of stress. In a certain range of intensity and frequency, the stress caused a significant changes to cell cycle paraments and an increase of the proportion of cells in S phase was seen, while the stress with higher frequency or intensity may cause the proportion of cells in S phase decreased evidently.

关 键 词：流式细胞术 交变应力 细胞周期 烟草细胞 植物 生长 强声波 

分 类 号：Q942.7[生物学—植物学]