甲低对新生早期大鼠海马及齿状回Goα mRNA的影响  

EFFECTS OF HYPOTHYOIDISM ON GOα GENE EXPRESSION IN HIPPOCAMPUS AND DENTATE GYRUS DURING EARLY DEVELOPMENT OF RAT BRAIN

作　　者：蔡东升[1] 陈源[1] 陈刚[1] 罗敏[1] 

机构地区：[1]上海第二医科大学附属瑞金医院,上海市内分泌研究所上海200025

出　　处：《中国应用生理学杂志》2001年第1期21-24,共4页Chinese Journal of Applied Physiology

摘　　要：目的 :研究甲状腺功能低下 (甲减 )对围生早期大鼠海马及齿状回GoαmRNA表达的影响。方法 :采用地高辛标记寡核苷酸探针原位杂交技术 ,观察 7d龄围生期甲减及正常Wistar大鼠海马CA1 4区及齿状回GoαmRNA的表达状况。结果 :7d龄甲减鼠脑海马各区及齿状回GoαmRNA的表达明显高于对照组 ,以CA3、CA4最为显著。结论 :在鼠脑发育过程中 。Aim:To study the expression of Goα mRNA in hippocampus and dentate gyrus of 7 day rats with hypothyroidism.Methods:We use wistar rat model of hypothyroidism and non radioactive in situ hybridization technique to observe the changs of Goα mRNA levels in hippocampus and dentate gyrus of 7 day rats with or without hypothyroidism.Results:Perinatal hypothyroidism can increase Goα mRNA levels in all areas of hippocampus and dentate gyrus of 7 day rats.Conclusion:Thyroid hormone can down regulate Goα gene transcription in hippocampus and dentate gyrus during the early development of rat brain.

关 键 词：甲状腺功能低下 原位杂交 大鼠 Goα 新生儿 

分 类 号：R581.2[医药卫生—内分泌] R722[医药卫生—内科学]