Caveolin-1促进糖尿病大鼠缺血下肢血管形成的作用及机制  

The effects and mechanism of Caveolin-1 on limb ischemia region of diabetic rat

作　　者：熊国祚[1] 刘辉[2] 李天平[3] 颜亚平[1] 申昕[1] 戴先鹏[1] 邓礼明[1] 毕国善[1] 胡兵兵[1] 

机构地区：[1]南华大学附属第二医院血管外科,湖南衡阳421001 [2]湖南省益阳市中心医院,湖南益阳413000 [3]南华大学药物药理研究所,湖南衡阳421001

出　　处：《中国当代医药》2014年第18期4-8,共5页China Modern Medicine

基　　金：湖南省教育厅科学研究青年基金(14JJ1024);湖南省高校创新平台开放基金(12K096);湖南省教育厅科学研究基金(09C845)

摘　　要：目的探讨Caveolin-1对下肢缺血糖尿病大鼠缺血部位血管形成的作用及与AKT信号通路的关系。方法健康、雄性SD大鼠,在腹腔注射链脲菌素构建的1型糖尿病大鼠模型上离断左侧股动脉及其分支,建立急性下肢缺血动物模型,14 d后用Western blot检测Caveolin-1、AKT的表达水平;28 d后进行CD34免疫组织化学染色和HE染色,评价血管密度。结果缺血组和糖尿病组与假手术组相比,Caveolin-1蛋白的表达水平明显增加(P<0.05);且缺血组Caveolin-1蛋白含量显著高于糖尿病组(P<0.05)。缺血组、缺血转染组、缺血空转染组的Caveolin-1蛋白、AKT水平均明显高于假手术组(P<0.05);缺血转染组与缺血组相比,Caveolin-1蛋白、AKT的表达水平明显增多(P<0.05)。与假手术组相比,缺血组、缺血转染组和缺血空转染组的微血管均有一定程度增加,其中缺血转染组的微血管密度增加尤为显著(P<0.05)。结论 Caveolin-1过表达后对糖尿病大鼠下肢缺血部位的血管形成有明显的促进作用,可能与激活AKT有关。Objective To investigate the function of Caveolin-1 on angiogenesis in lower limb ischemia region of dia-betic rat and the relationship with AKT signal pathway. Methods Intraperitoneal injection of healthy,male,SD rats with streptozocin to construct type 1 diabetic rat model,then broken the left femoral artery to set up the acute lower limb is-chemia animal model.After 14 days with Western blot detection Caveolin-1 and AKT expression.CD34 immunohisto-chemical staining and HE staining was applied to detect the vascular density in 28 days. Results The level of Cave-olin-1 in diabetes group and ischemia group was obviously higher than that in sham group respectively,the level of Caveolin-1 in ischemia group was higher than that in diabetes group,with statistical difference （P〈0.05）.The level of Caveolin-1 and AKT in ischemia group,ischemic transfection group,ischemic idling transfected group was obviously higher than that in sham group respectively,the level of Caveolin-1 and AKT in ischemic transfection group was obvi-ously higher than that in ischemia group respectively,with statistical difference（P〈0.05）.Compared with the sham opera-tion group,microvascular had a certain degree increased in ischemia group,ischemia transfection group and ischemic idling transfected group,the microvessel density in the ischemic transfection group increased significantly （P〈0.05）. Conclusion The higher expression of Caveolin-1 in diabetic lower limb ischemia in rats has obvious promoting effect to its angiogenesis,this effect may be related to activation of AKT.

关 键 词：CAVEOLIN-1 糖尿病 下肢缺血 

分 类 号：R587.1[医药卫生—内分泌]