卫星搭载红豆草后代中耐盐细胞系的筛选及鉴定  

SELECTION AND IDENTIFICATION OF SALT TOLERANT LINE OF SAINFOIN FROM THE SEEDS OF FIRST POST-FLIGHT PLANTS

作　　者：徐云远[1] 王鸣刚[2] 贾敬芬[3] 

机构地区：[1]中国科学院植物研究所,北京100093 [2]厦门大学生物系,厦门361005 [3]西北大学生物系,西安710069

出　　处：《实验生物学报》2001年第1期11-15,共5页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金(No.39770365)资助

摘　　要：将红豆草种子搭载于940703返地卫星,经田间繁殖得后代种子,先将种子在1.5%NaCl上筛选、并在该盐浓度下诱导愈伤组织和筛选,在无盐培养基上恢复生长后再在1.2%NaCl上筛选得到耐盐变异系。变异系具有正常的分化能力并表现出对PEG胁迫的交叉抗性。变异系在无胁迫条件下脯氨酸含量较低但在有盐胁迫时具有高效积累脯氨酸的能力。后者可能对红豆草耐盐系更为重要。变异系中脯氨酸的这种合成机理可能是由于一些基因在调控中对水的敏感性改变引起。梯度聚丙烯酰胺凝胶电泳表明耐盐系的SOD和酯酶分别出现175kD和75kD的新形式。说明空间诱变和组织培养相结合可以筛选耐盐变异系。Seeds of sainfoin (Onobrychis vicaefolia Scop. ) were carried in the recoverable satellite 940703 and recovered from earth orbit from China in 1994. The progeny seeds were obtained by producing in field. The salt tolerant calluses were selected by screening seedling and callus on 1.5% NaCl-containing medium , reviving growth on Nad- free medium and selecting callus on 1.2% NaCl-containing medium. The salt tolerant line callus maintained the normal ability to regenerate plant. The salt tolerant line callus exhibited cross-resistance to PEG stress. The variant appeared higher efficiency than control to accumulate proline under salt stress, however, under nous tress condition it had lower proline level than control, which suggested that the higher efficiency to synthesize proline under stress condition may be more important than higher level in tissue under nonstress condition. The mechanism of proline synthesis in the selected callus was considered to result from the alteration in gene sensitivity to water regulation at transcription level. Acrylamide gradient electrophoresis showed that new isoen/yme form with MW175kD and 75kD of superoxide dismutase and esterase respectively appeared in salt torlerant callus. It was indicated that the combination of space mutagenesis with tissue culture could be used for the selection of salt tolerani sainfoin line in vitro.

关 键 词：红豆草 卫星搭栽 耐盐细胞系 牧草 选育 

分 类 号：S54[农业科学—作物学]