液质联用在多肽及蛋白质定性方面的研究进展  

Applications of Liquid Chromatography-Mass Spectrometry in the Qualitative Analysis of Peptides and Proteins

作　　者：范学海[1] 朱颐申[1] 陈兰婷 李文惠[1] 韦萍[1] 

机构地区：[1]南京工业大学生物与制药工程学院,南京211816

出　　处：《生物技术通报》2014年第6期62-66,共5页Biotechnology Bulletin

基　　金：江苏高校优势学科建设工程资助项目

摘　　要：随着液质联用技术的不断发展,液相色谱—质谱联用系统广泛应用于药物分析、食品分析、环境分析等多方面,该技术将液相色谱的高分离能力与质谱强大的结构鉴定功能相结合,具备了高分离度、高灵敏度、高选择性以及提供丰富结构信息等一系列优点。这些优点决定了液相色谱—质谱联用系统将在越来越多地相关领域得到应用,尤其是近年来在生物大分子方面开展了大量研究,结合这些研究对液质联用技术在多肽及蛋白质定性方面进行了系统归纳。With liquid chromatography-mass spectrometry（LC-MS）technology developing continuously, the system of LC-MS is widely used in pharmaceutical analysis, food analysis, environmental analysis and related fields. LC-MS provides powerful function of structure identification, which includes the ability of separation by LC and the advantages of high resolution, high sensitivity, high selectivity by MS. These advantages demonstrate that LC-MS will be applied in more related fields. In recent years, many researches were carried out in the identification of biological macromolecules by LC-MS. The analysis of peptide and protein qualitatively by LC-MS is reviewed.

关 键 词：液质联用(LC-MS) 多肽和蛋白质 定性分析 

分 类 号：Q51[生物学—生物化学]