北五味子愈伤组织增殖及胚性愈伤组织诱导的研究  

The Study of Callus Proliferation and Embryogenic Callus Induction of Schisandrach chinesis (Turcz.) Baillon

作　　者：李千[1] 孙丹[1] 李宏博[1] 张舞曲 朴钟云[1] 

机构地区：[1]沈阳农业大学园艺学院,辽宁沈阳110866

出　　处：《种子》2014年第6期5-9,共5页Seed

基　　金：农业部公益性行业科研专项(编号:201103037)

摘　　要：目的:探讨不同因素对北五味子愈伤组织增殖以及胚性愈伤组织诱导的效应。方法:以MS为基本培养基,研究不同植物生长调节剂、继代次数、蔗糖浓度以及光照长度对愈伤组织增殖的影响;不同植物生长调节剂诱导胚性愈伤组织的效应。结果:北五味子愈伤组织增殖率最高的培养基是附加2,4-D 1.0 mg/L+6-BA 0.5 mg/L,适宜的继代次数是2次,蔗糖浓度为30 g/L,光照长度15 d增殖率最高;胚性愈伤组织诱导率最高的是附加TDZ1.0 mg/L+ZT0.2 mg/L。结论:高浓度的生长素、低浓度的细胞分裂素适宜愈伤组织的增殖;继代次数增多、光照长度延长、蔗糖浓度过高过低都不适宜愈伤组织增殖。Objective：Discussed the effect of different factors on the callus proliferation and embryogenic callus induction of Schisandrach chinesis （Turcz.） Baillon.Method：The effect of MS supplied with different plant growth regulators,subculture number,sugar concentrarion and illumination length on callus proliferation and different plant growth regulators on embryogenic callus induction were studied.Result：MS ＋ 2,4-D 1.0 mg/L ＋ 6-BA 0.5 mg/L supplied with 30 g/L sugar and illuminating for 15 d was best for the callus proliferation;the best subculture time was the secondly ; MS supplied with TDZ 1.0 mg/L ＋ ZT 0.2 mg/L was beneficial to embryogenic callus induction.Conclusion：High concentration auxin and low concentration cytokinin was suitable for callus proliferation; subculture number increase,illumination time extension and the sucrose concentration too high or too low were all not suitable for callus proliferation.

关 键 词：北五味子 愈伤组织 增殖 胚性愈伤组织 

分 类 号：S567.19[农业科学—中草药栽培]