利用等位酶对油松无性系种子进行父本分析  

A PATERNITY ANALYSIS OF SEEDS FROM DIFFERENT CLONES IN A PINUS TABULAEFORMIS CARR. SEED ORCHARD

作　　者：张冬梅[1] 沈熙环[1] 何田华[2] 

机构地区：[1]北京林业大学,北京100083 [2]中国科学院植物研究所系统与进化植物学开放研究实验室,北京100093

出　　处：《植物生态学报》2001年第2期166-174,共9页Chinese Journal of Plant Ecology

基　　金：高等学校博士学科点科研基金! (970 0 2 2 0 8)

摘　　要：利用水平淀粉凝胶电泳 8种酶 10个位点 34个等位基因 ,对辽宁兴城油松无性系种子园中 17个无性系2 0个单株的 2 0 0粒种子作了父本分析。无性系雄花量的多少与授粉座果率并不成正比 ;不同无性系对花粉的接受存在一定的选择性 ,分析试样单株与周围的无性系并没有构成固定的授粉组合 ,说明授粉期风向等气象因子 ,无性系的雌雄花器的构造 ,受精机理等对受粉的成败都有作用 ;种子园结实初期自交现象并不明显 ,在分析试样中只有16 # ,17# ,19#无性系存在自交现象 ;种子园内花粉的有效传播一般在半径 30 m范围内。同时 ,分析了种子园子代的等位酶变异和空间遗传结构 ,从另一侧面反映出采种母树周围花粉分布比较均匀 。Paternity analysis of a clonal seed orchard of Pinus tabulaeformis Carr. was conducted by means of allozyme markers. Multilocus allozyme genotypes at 10 loci of 17 clones were inferred from a sample of at least eight megagametophytes per clone, while the genotypes of the other 32 parental clones were from buds. A total of 200 embryos and endosperms were analyzed, of which the paternity could be accurately identified for just 89 samples. A low degree of selfing was found within the seed orchard, while higher male strobili production did not indicate a higher degree of fertilization success. Half of the allozyme loci were found to deviate from Hardy-Weinberg equilibrium. At the same time, the effective dispersal distance of pollen and spatial genetic structure of the seed orchard population were analyzed by means of spatial autocorrelation. A few Moran's I values for this test in short distance classes were significantly positive. The results indicated that the pattern of spatial genetic structure is very weak in the progeny population.

关 键 词：油松 种子园 等位酶 父本分析 空间自相关 遗传结构 

分 类 号：Q346[生物学—遗传学]