鸡毒支原体F弱毒疫苗株细胞免疫特性研究  

THE EFFECT OF MYCOPLASMA GALLISEPTICUM F VACCINE ON CELLULAR IMMUNITY

作　　者：林伯全[1] 徐磊[1] 杨慧[1] 林秋敏[1] 

机构地区：[1]福建农业职业技术学院动物科学系,福州350119

出　　处：《中国动物传染病学报》2014年第3期78-81,共4页Chinese Journal of Animal Infectious Diseases

基　　金：新世纪"百千万人才工程"国家级人选补助基金项目(NCNCMTPC-2009);2011年福建省教育厅A类科技项目(JA11320);2012年福州市科技计划项目(2012-N-94)

摘　　要：为了解鸡毒支原体(Mycoplasma gallisepticum,MG)F弱毒疫苗株的细胞免疫特性,分别以活菌浓度为109(A组)、106(B组)ccu/mL的MG F株及生理盐水(C组)点眼接种SPF鸡,采用流式细胞技术及T淋巴细胞增殖试验对免疫前后淋巴细胞亚类Th/T、Tc/T、Th/Tc及刺激指数(SI)的动态变化规律进行研究。结果显示免疫后A、B组的Th/T、Tc/T、SI明显升高,其中Th/T于d5、d7,Tc/T、SI于d7、d14,A组显著高于B组(P<0.05)。研究结果表明,免疫MG F株可较好的提高鸡的细胞免疫,且MG F株活菌浓度与接种鸡外周血Th/T、Tc/T、SI呈正相关。To evaluate the effect of Mycoplasma gallisepticum （MG） F vaccine on cellular immunity, SPF chickens were inoculated with 109 ccu/mL MG （A group）, 106 ccu/mL MG （B group） and saline （C group）, respectively. In addition, the change of Th/T, Tc/T, Th/Tc and SI were analyzed by Flow cytometry and lymphocytes proliferation test. The results showed that Th/T, Tc/T, Th/Tc and SI of A and B group increased significantly. Both Th/T on 5 and 7 days post vaccination （dpv） and Tc/T, SI on 7 and 14 dpv of A group were significantly higher than B group （P〈0.05）. These findings indicated that MG F vaccine was able to enhance the cellular immune response and Th/T, Tc/T and SI in peripheral blood mononuclear cells （PBMCs） were positively correlated with the inoculation close of MG F vaccine.

关 键 词：鸡毒支原体 F弱毒疫苗 细胞免疫 淋巴细胞亚类 淋巴细胞增殖 

分 类 号：S852.62[农业科学—基础兽医学]