我国中华按蚊群体分子遗传多态研究  

Study on molecular genetic polymorphism of Anopheles sinensis populations in China

作　　者：雅军 瞿逢伊[1] 徐建农[1] 郑哲民[2] 

机构地区：[1]第二军医大学基础部寄生虫学教研室,上海200433 [2]陕西师范大学生命科学学院,西安710062

出　　处：《昆虫学报》2001年第1期33-39,共7页Acta Entomologica Sinica

基　　金：国家自然科学基金资助项目!(396 70 6 6 3)

摘　　要：利用RAPD PCR技术研究了采自我国 9省 10个代表点的中华按蚊Anophelessinensis自然群体的遗传多态现象 ,根据 2 3个RAPD等位基因位点进行了分析。结果显示 :①多态位点比例为6 8 2 %～ 86 4 % ,期望平均杂合度为 0 2 4 9～ 0 348,说明中华按蚊群体具广泛的遗传多态现象 ;②利用 3种方法计算Fst和θ,平均值为 0 0 6 9～ 0 111,相应的迁移率Nm为 2 0～ 3 4 ,表明基因流水平较低 ;③中华按蚊各自然群体间的遗传一致性达 0 8795～ 0 9973,平均遗传距离为0 0 4 1± 0 0 33,属种内变异范围。聚类分析显示 ,群体间遗传距离与地理位置无对应关系。Samples of Anopheles sinensis from 10 collection sites in 9 provinces of China were analyzed for genetic polymorphism using RAPD PCR. Twenty three RAPD loci were studied and the results were as follows:① The percentage of polymorphic loci was 68 2%～86 4%, and the expected mean heterozygosity was 0 249～0 348, which indicated extensive genetic polymorphism in the natural populations of An.sinensis. ② The \%Fst\% and θ were caculated using 3 measures. Mean values were 0 069～0 111 with corresponding migrating rate of 2 0～3 4. It indicated that the level of gene flow was low. ③The genetic identity among the natural populations was 0 8795～0 9973 with the mean genetic distance of 0 041±0 033. The data are within the range of intraspecific variation. Cluster analysis revealed that the genetic distance did not relate to the collection sites.

关 键 词：中华按蚊 群体 分子遗传多态 

分 类 号：Q969.442.2[生物学—昆虫学]