表皮生长因子对卵泡刺激素促进的小鼠胚胎卵巢卵泡发育和雌二醇分泌的影响  

Effects of Epidermal Growth Factor on the Follicle Development and Estradiol Secretion of Mouse Fetal Ovary Induced by Follicle-Stimulating Hormone in vitro

作　　者：王海滨[1] 夏国良[1] 李美玲[1] 郭勇[1] 谢辉蓉[1] 

机构地区：[1]中国农业大学生物学院,北京100094

出　　处：《农业生物技术学报》2001年第1期37-40,共4页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金资助(编号39770396)

摘　　要：：ＦＳＨ、ＥＧＦ是参与卵巢功能调节的重要因素。关于ＥＧＦ对ＦＳＨ促进的胚胎卵巢卵泡发育和类固醇激素发生的影响报道甚少。本实验以单个卵巢的总卵泡数、生长卵泡数和５个最大卵母细胞的平均直径为卵泡发育优劣的衡量指标，利用我们早期研究建立的无血清培养体系研究了ＥＧＦ对ＦＳＨ促进的小鼠胚胎卵巢卵泡发育和分泌雌二醇的作用。结果发现：①ＥＧＦ＋ＦＳＨ＋雄烯二酮（Ａ）处理组的胚胎卵巢总卵泡数在培养第 ７～９ｄ显著高于ＦＳＨ＋Ａ处理组，而且生长卵泡数和卵巢内５个最大卵母细胞平均直径在培养后期也显著高于ＦＳＨ＋Ａ处理组（Ｐ＜０．０５）；②ＦＳＨ＋Ａ处理组的胚胎卵巢在培养第７～９ｄ分泌大量雌二醇，而ＥＧＦ和ＦＳＨ联合使用时胚胎卵巢在培养早期雌二醇分泌水平显著低于ＦＳＨ＋Ａ处理组（Ｐ＜０．０５）。结果提示ＥＧＦ协同ＦＳＨ促进小鼠胚胎卵巢卵泡体外生长发育；同时ＥＧＦ抑制ＦＳＨ诱导的雌二醇分泌，其促卵泡发育作用可能与雌二醇的合成不相关联。Follicle-stimulating hormone (FSH) and Epidermal Growth Factor (EGF) are important regulatory proteins, which involve the regulation of ovarian function. Very few informations were reported on the regulation of EGF in follicle development and steroidogenosis of fetal ovary. The present experiment was conducted to evaluate the regulation of EGF on the FSH-induced follicular development and estradiol secretion of mouse fetal ovary in a serum-free condition. The average number of all oocytes, the growing oocyte per-ovary and the average diameter of the five largest growing oocytes were applied as the index of follicular development. The results revealed that: ① The total number of oocyte in EGF+FSH+A group was significantly higher than that in FSH+A group between day 7 to 9 in the culture period (P<0.05). The growing oocyte number and the average diameter of the five lapest oocytes in EGF+ FSH+A group were significantly prior to that in FSH+A group in the late culture period (P<0.05). ② A higher level of estradiol in FSH+A group was detected from 7 to 9 culture days, While at the same period only low concentration of estradiol existed in EGF+FSH+ A group. The results showed that EGF had a synergic role with FSH in follicular development of mouse fetal oval but Which attenuates the production of estradiol induced by FSH in vitro. This suggested that the stimulatory effect of EGF on follicular development is independent of the estradiol production.

关 键 词：小鼠 胚胎卵巢 卵泡发育 表皮生长因子 卵泡刺激素 雌二醇 

分 类 号：Q492.5[生物学—生理学] Q954.432