慢性氟中毒大鼠骨组织中G蛋白的表达  

Expression of G protein in bone tissue of rats with fluorosis

作　　者：吴延莉 向蓉[1] 徐小东[1] 曹发军[1] 范祥[1] 韦艳[1] 

机构地区：[1]贵阳医学院公共卫生学院环境卫生学教研室,贵州贵阳550004

出　　处：《环境与健康杂志》2014年第4期296-299,共4页Journal of Environment and Health

基　　金：国家自然科学基金(81360412);贵州省科技厅社会发展攻关计划项目(黔科合SY字[2013]3062号)

摘　　要：目的观察慢性氟中毒大鼠骨组织中激动型G蛋白(Gs)和抑制型G蛋白(Gi)含量的变化。方法将48只断乳2周的Wistar大鼠按体重随机分为4组,分别为对照(自来水)组和50、150、250 mg/L NaF染毒组,每组12只,雌雄各半。采用自由饮用的方式进行染毒,连续染毒6个月。检测大鼠尿氟、骨氟含量及氟斑牙发生率以及骨组织中Gs和Gi的水平。结果与对照组比较,各剂量NaF染毒组大鼠骨氟含量及雄性大鼠尿氟含量以及150、250 mg/L NaF染毒组雌性大鼠尿氟含量均较高,各剂量NaF染毒组大鼠氟斑牙的检出率也均较高,仅150 mg/L NaF染毒组雌性大鼠骨组织中Gs含量以及雄性大鼠骨组织中Gs、Gi含量均较高,差异有统计学意义(P<0.05);各剂量NaF染毒组大鼠骨组织中Gi/Gs值无明显变化。随着NaF染毒剂量的升高,大鼠的尿氟、骨氟含量均呈上升趋势;而骨组织中Gs、Gi含量均呈先升高后下降的趋势。对照组大鼠无氟斑牙发生,而各剂量NaF染毒组大鼠氟斑牙的检出率均为100%。结论 G蛋白在氟骨症的形成过程中可能发挥一定的促进作用。Objective To observe the changes of the content of stimulating adenylate cyclase G protein（Gs） and inhibitory adenylate cyclase G protein（Gi） in bone tissue of rats with fluorosis. Methods Forty-eight Wistar rats weaned four weeks were randomly divided into four groups according to body weight,control group（treated with tap water） and NaF exposure groups（treated with NaF at 50,150,250 mg/L）,six female rats and six male rats in each group. NaF was given through drinking water. After six months of treatment, the bone and urinary fluoride content and incidence rate of dental fluorine and the levels of bone tissue Gs and Gi were determined. Results Compared with the control, bone fluoride of the rats in each experimental group, urinary fluoride of the male rats in each experimental group and urinary fluoride of the female rats treated with 150 and250 mg/L NaF were obviously higher,and the incidence of dental fluorosis in each NaF exposure group was also significantly higher（P〈0.05）. Only the content of bone tissue Gs of female rats and the content of bone tissue Gs and Gi of the male rats treated with 150 mg/L NaF were significantly higher than those of control（P〈0.05）. No significant differences were found in Gi/Gs in bone tissue among all groups. Urinary and bone fluoride contents of the rats showed an upward trend with increasing NaF dose;The content of bone tissue Gs and Gi showed a downward trend after an initial increase. The incidence of dental fluorosis of each NaF exposure group was 100% and dental fluorosis was not found in control. Conclusion G protein may promote the formation of skeletal fluorosis to a certain degree.

关 键 词：G蛋白 慢性氟中毒 骨组织 

分 类 号：R994.6[医药卫生—毒理学]