大鼠旁巨细胞外侧核呼吸神经元的类型及其对某些递质类药物的反应  

THE TYPES OF RESPIRATORY NEURONS IN NEUCLEUS PARAGIGANTOCELLULARIS LATERALIS IN RATS AND THEIR RESPONSES TO SOME MEDICINES

作　　者：李莉华[1] 郑煜[2] 徐美丽[2] 

机构地区：[1]泸州医学院生理教研室,泸州646000 [2]华西医科大学生理教研室,神经生理研究室,成都610041

出　　处：《中国应用生理学杂志》2001年第1期10-13,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助课题!(390 70 36 9)

摘　　要：目的和方法 :用细胞外记录法和多管微电极微电泳法在 36只麻醉自主呼吸的SD大鼠 ,系统探查旁巨细胞外侧核尾半侧 (cPGCL)呼吸神经元 (RNs)的类型及该区的递质受体情况。结果 :在 14只大鼠系统探查 ,记录到 39个RNs ,包括吸气神经元 2 4个、呼气神经元 12个和跨时相神经元 3个。在另 2 2只大鼠 ,观察到微电泳谷氨酸钠 (L Glu)使 12 / 14个RNs兴奋 ,γ 氨基丁酸 (GABA)使全部受试RNs抑制 (n =2 2 ) ;对NMDA受体拮抗剂DL 2 氨基 5 磷酸戊酸 (AP5)、GABAA 受体拮抗剂荷包牡丹碱 (BIC)有兴奋、抑制和无作用三类效应 ;AP5部分阻断大部分受试神经元 (6 / 9个 )对L Glu的兴奋反应 ,BIC部分或完全阻断大部分受试神经元 (9/ 11个 )对GABA的抑制反应。结论 :PGCL是呼吸调控的重要中枢部位之一 ,该区可能存在起神经递质作用的内源性L Glu和GABA及兴奋性氨基酸 (包括NMDA和非NMDA)受体和GABAA 受体 。Aim and Methods: In 36 anesthetized spontaneously breathing Sprague-Dawley rats, the types of respiratory neurons and the state of neurotransmitters and receptors in nucleus paragigantocellularis lateralis (PGCL) were studied with extracellular recording technique and multibarrel microelectrode techniques.Results:At the caudal part of the PGCL(cPGCL) in 14 rats, a total of 39 respiratory neurons(RNs) were recorded including 24 inspiratory, 12 expiratory and 3 phase spanning neurons.At the cPGCL in another 22 rats, we discovered that out of 14 RNs tested,12 were excited by iontophoretic application of sodium glutamate (L-GLu), and all the 22 RNs tested were inhibited by γ-aminobutyric acid (GABA).DL 2 amino 5 phosphonovaleric acid (AP 5), an antagonist of N-methyl-D-aspartate (NMDA)receptor, and bicuculline(BIC), an antagonist of GABA A receptor, both showed three kinds of effects on the RNs discharge: excitatory, inhibitory and no effect.AP 5 could block partially the excitatory effect of L GLu on a large part of RNs tested (6/9). BIC blocked, partially or completely, the inhibitory effect of GABA also on a large part of the RNs tested (9/11). Conclusion: It is implied that PGCL is one of the important neural substrates responsible for the regulation of respiration. There might exit endogenous glutamate and GABA acting as neurotransmitters in the cPGCL area and exicitatory amino acids (inclu ding NMDA and non-NMDA) and GABA A receptors on cPGCL neurons. These neurotransmitters and receptors may mediate the regulatory action of cPGCL on respiration.

关 键 词：旁巨细胞外侧核 呼吸神经元 谷氨酸 Γ-氨基丁酸 大鼠 递质类药物 

分 类 号：R338[医药卫生—人体生理学]