104份甘肃小麦品种脂肪氧化酶和多酚氧化酶活性基因等位变异的检测  

Molecular Detection of Allelic Variations of Genes for Lipoxygenase and Polyphenol Oxidase in 104 Gansu Wheat Cultivars

作　　者：曹东[1,2] 张雪婷[3] 王世红[3] 王东霞[1,2] 赖勇[1,2] 孟亚雄[1,2] 王化俊[1,2] 杨文雄[3] 

机构地区：[1]甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070 [3]甘肃省农业科学院小麦研究所,甘肃兰州730070

出　　处：《麦类作物学报》2014年第4期467-473,共7页Journal of Triticeae Crops

基　　金：国家自然科学基金项目(30771331;31171558);甘肃省科技重大专项(1203NKDF018);甘肃省自然科学基金项目(1208RJ2A136)

摘　　要：面粉和面制品的色泽是评价小麦品质的重要指标。小麦脂肪氧化酶(LOX)和多酚氧化酶(PPO)活性对面粉白度及面制品的色泽具有重要影响。为给甘肃省小麦品质育种提供参考依据,以104份甘肃省育成的小麦品种为材料,利用功能标记LOX16、LOX18、PPO18、PPO16和PPO29检测TaLox-B1、PpoA1及Ppo-D1位点的等位变异,分析甘肃小麦品种资源中LOX和PPO活性基因的组成和分布特点。结果表明,在甘肃小麦中,等位变异TaLox-B1a和TaLox-B1b的频率分别为22.12%和77.88%;其中,甘肃冬小麦品种高LOX活性等位变异TaLox-B1a的分布频率(30.56%)高于春小麦(3.13%)。等位变异Ppo-A1a、PpoA1b、Ppo-D1a和Ppo-D1b的频率分别为49.04%、50.96%、50.96%和49.04%;两个PPO基因的等位变异组合Ppo-A1a/Ppo-D1b、Ppo-A1a/Ppo-D1a、Ppo-A1b/Ppo-D1b和Ppo-A1b/Ppo-D1a的分布频率依次为28.85%、20.19%、20.19%和30.77%。说明在甘肃小麦品种中,低LOX活性等位变异(TaLox-B1b)品种比例较高;低PPO活性等位变异(Ppo-A1b、Ppo-D1a)品种分布比例略高于高PPO活性类型;其中,32份小麦品种在TaLox-B1、Ppo-A1和Ppo-D1三个位点同时含有高LOX活性和低PPO活性的等位变异。Flour color and appearance color of flour products are the important indicators for evaluating wheat quality.The activities of wheat lipoxygenase（LOX）and polyphenol oxidase（PPO）have relatively important effects on the flour whiteness and color of flour products.In this study,104wheat varieties released in Gansu province were used as experimental materials to detect the allelic variation of lipoxygenase gene TaLox-B1 and polyphenol oxidase gene Ppo-A1 and Ppo-D1 by using function marker LOX16,LOX18,PPO18,PPO16 and PPO29,in order to further investigate the distribution characteristics of the three genes in wheat varieties from Gansu.The results showed that,the fre-quencies of cultivars with genes of TaLox-B1aand TaLox-B1b were 22.12%and 77.88%,respectively;the distribution frequency of LOX high activity gene TaLox-B1ain winter wheat varieties from Gansu（30.56%）was higher than that in spring wheat（3.13%）.The frequencies of cultivars with genes of Ppo-A1a,Ppo-A1b,Ppo-D1aand Ppo-D1b were 49.04%,50.96%,50.96% and 49.04%, respectively.Frequencies of allelic combinations of PPO genes for Ppo-A1a/Ppo-D1b,Ppo-A1a/ Ppo-D1a,Ppo-A1b/Ppo-D1band Ppo-A1b/Ppo-D1awere 28.85%,20.19%,20.19%and 30.77%, respectively.Wheat cultivars recently released in Gansu province had a relatively high proportion of LOX low-activity gene TaLox-B1b.and the genes of low PPO activity were dominant.Among those varieties,32varieties contained the desired allelic variation for LOX and PPO genes.

关 键 词：甘肃小麦 脂肪氧化酶 多酚氧化酶 等位变异 功能标记 

分 类 号：S512.1[农业科学—作物学] S331