四川小麦品种(系)淀粉颗粒结合蛋白多态性分析  

Genetic Diversity of Starch Granule Associated Proteins in Landraces and Cultivars(Lines)of Sichuan

作　　者：刘安军[1] 杨晓怡[1] 刘永红[1] 盛玉珍[1] 陈国跃[2] 蒲至恩[1,2] 刘亚西[2] 李伟[1,2] 

机构地区：[1]四川农业大学农学院,四川成都611130 [2]四川农业大学小麦研究所,四川成都611130

出　　处：《麦类作物学报》2014年第4期474-481,共8页Journal of Triticeae Crops

基　　金：四川省人力资源与社会保障厅留学回国人员择优资助项目

摘　　要：为了解四川小麦品种(系)淀粉颗粒结合蛋白的多样性,对66份四川小麦地方品种、100份新育成品种(系)以及21份小麦骨干亲本材料的淀粉颗粒结合蛋白进行了SDS-PAGE分析。结果表明,在187份供试材料中共分离出7种蛋白条带组合类型,10种不同的蛋白条带,其中5种条带在供试材料中检测到缺失和迁移率改变。114份材料表现为SGP-1A蛋白缺失,而在所有供试的四川小麦地方品种中该蛋白均表达。共4份材料在SGP-1B位点蛋白迁移率改变,18份材料的Wx-1B蛋白条带缺失,8份材料的Wx-1D蛋白条带缺失,仅有1份材料的Wx-1A蛋白条带缺失。通过与中国春缺体-四体(N7AT7B、N7DT7B、N4AT4B)、乌拉尔图小麦(AA)、节节麦(DD)、圆锥小麦(AABB)淀粉颗粒结合蛋白的比较,并结合质谱鉴定,进一步验证了所鉴定条带的准确性。本研究表明,四川小麦新育成品种(系)和骨干亲本比四川小麦地方品种具有更丰富的淀粉颗粒结合蛋白变异类型,鉴定出的变异材料可用于淀粉颗粒结合蛋白功能的研究和小麦淀粉品质育种。In order to know the genetic diversity of wheat starch granule-associated proteins（SGAPs） in Sichuan wheat,66wheat landraces,100new wheat cultivars（lines）and 21wheat founder parents from Sichuan were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis（SDSPAGE）.A total of seven combinations and ten different protein bands were identified in the 187materials,and the variations,including unexpressed and the change of mobility,were observed on five bands.The null SGP-1A,the null Wx-1B,and the null Wx-1Dwere detected in 114,18,and 8materials,respectively,but the null Wx-1A was only observed in one material.Based on the comparison with SGAPs in Chinese Spring nullisomic tetrasomic lines（N7AT7B,N7DT7B,N4AT4B）,and T. urartu（AA）,Ae.tauschii（DD）,T.turgidum（AABB）,and the results of MALDI-TOF-MS,we further confirmed that the identification of SGAPs was correct.Our result suggested that the new varieties（lines）showed higher diversity on SGAPs than the landraces of Sichuan,and the materials with the variation of SGAPs could be used in the functional research of SGAPs and the wheat breedingon starch quality.

关 键 词：小麦 淀粉颗粒结合蛋白 多态性 

分 类 号：S512.1[农业科学—作物学] S331