急性运动所致线粒体某些功能的改变及胆红素的保护作用  

CHANGES OF THE CERTAIN FUNCTIONS OF MITOCHONDRIA INDUCED BY ACUTE EXERCISE AND THE PROTECTIVE EFFECT OF BILIRUBIN

作　　者：韩春华[1] 王起恩[2] 王生[2] 沈惠麒[2] 

机构地区：[1]首都医科大学预防医学系,北京100054 [2]北京医科大学劳动卫生教研室,北京100083

出　　处：《中国应用生理学杂志》2001年第1期72-75,共4页Chinese Journal of Applied Physiology

摘　　要：目的 :探讨急性运动所致疲劳的机理以及胆红素的保护作用。方法 :将Wistar大鼠随机分为对照组、运动组、运动恢复组、胆红素处理运动组、胆红素处理运动恢复组 ,共 5组 ,分别灌胃 1μmol/Kg体重的胆红素或生理盐水 4周 ,负重 (体重的 5 % )游泳 2h后处死 ,测定有关指标。结果 :急性运动后即刻大鼠腓肠肌胞浆、线粒体Ca2 +含量明显升高 ,恢复 12h后线粒体Ca2 +含量呈继续升高的趋势 ;线粒体Ca2 + Mg2 + ATP酶、Ca2 + ATP酶活性明显下降 ,12h后有所回升 ;胆红素处理后可以明显抑制这些指标的改变 ,但线粒体Mg2 + ATP酶活性的变化在胆红素处理组和未处理组间无明显差异 ,均明显低于对照组 ,只是胆红素组的恢复相对较快。结论 :生理浓度的胆红素可能通过抑制线粒体某些功能的改变而保护细胞免受急性运动所致的损伤 ,从而延缓疲劳的发生 。Aim: To study the mechanism of fatigue induced by acute exercise and the protective effect of bilirubin. Methods: 41 Wistar male rats were divided into five groups: control group,exercise group, exercise recovery group,bilirubin treateol exercise group anol bilirubin treateol exercise recovery group.The rats were administrated with 1 μmol/kg body weight of bilirubin or saline once every day for 4 weeks, after swimming with load for 2 h, all of the rats were killed and several index were determined. Results: Acute exercise could induce the increase of Ca 2+ content in cytoplasm and mitochondria of gastrocnemius, Ca 2+ content increased continuously after 12 hours in mitochondria. The ca 2+ Mg 2+ ATPase activity decreased obviously after acute exercise, and the activity increased again after 12 hours. Bilirubin could inhibit these changes. The change of Mg 2+ ATPase activity in mitochondria had little difference between bilirubin treated and untreated groups, both of which were lower than that of control group, but the recovery was faster in the bilirubin treated group than that in untreated group. Conclusion: Bilirubin could protect muscle fibers from injury induced by acute exercise and delay the development of fatigue and promote the recovery through inhibiting the disturbance of the certain function of mitochondria.

关 键 词：急性运动 胆红素 线粒体 CA^2+ C^a2+-Mg^2+-ATP酶 保护作用 

分 类 号：R87[医药卫生—运动医学]