蝎毒中枢镇痛机制的初步探讨  

PRELIMINARY STUDY OF ANALGESIC MECHANISM OF BUTHUS MARTANSII KARSCH(BmK) VENOM ON CENTRAL NERVOUS SYSTEM

作　　者：黄民[1] 潘玉贞[1] 毛华伟[2] 刘敏芝[1] 王绍[1] 

机构地区：[1]白求恩医科大学基础医学院生理教研室,长春130021 [2]长春税务学院卫生院,长春130022

出　　处：《中国应用生理学杂志》2001年第1期85-88,共4页Chinese Journal of Applied Physiology

摘　　要：目的 :研究BmK蝎毒是通过何种受体实现其中枢镇痛作用的 ;外侧隔核是否是BmK蝎毒产生中枢镇痛作用的重要部位之一。方法 :用辐射热一甩尾法测定痛阈 ,用玻璃微电极记录束旁核的单位放电 ;通过不锈钢套管向侧脑室和外侧隔核内微量注射 0 .0 1%BmK蝎毒。结果 :向大鼠侧脑室注射 2 μl 0 .0 1%蝎毒可明显升高痛阂。该效应可被侧脑室注射 2 μl 0 .2 5 %纳洛酮完全翻转 ;向隔核内注射 0 .5 μl 0 .0 1%Bmk蝎毒 ,分别对束旁核中的 71%(15 /2 1)痛兴奋单位和 83 % (5 /6 )痛抑制单位对痛刺激的反应减弱 ,而对痛无关单位的电活动无明显影响。结论 :BmK蝎毒的中枢镇痛作用可能主要是通过吗啡受体实现的 ;Aim: To investigate whether or not the lateral septal nucleus(LS) was one of important analgesic areas of Bmk venom in the central nervous system (CNS), and , targeting on which receptor,analgesic effect was produced by it. Methods: Pain threshold of skin was observed by the latent period of tail flick evoked by radiant heat. Glass micropipette placed in Pf was used to record unit dischanges of neurons in it, before and after 0.01% BmK venom were injected into LS. Stainless steel cannula placed in the lateral cerebral ventricle (icv) and LS was used for microinjection.Results: After injection of 2 μl 0.01% BmK venom into icv of rat,the pain threshold was aparently raised, which was completety returned by injection of 0.5 μl 0.25% naloxone into icv. After 0.5 μl 0.01% Bmk venom was injected into LS, 71%(15/21)nociceptive on neurons and 83.3%(5/6) nociceptive off neurons decreased the nociceptive response to tail pinch, but no evident effect was observed in the non nociceptive neurons. Conclusion:The analgesic effect of BmK venom was probably realized mainly by the opiate receptor, and LS was one of important analgesic areas of BmK venom on CNS.

关 键 词：BmK蝎毒 纳洛酮 镇痛 外侧隔核 

分 类 号：R971[医药卫生—药品] R996.3[医药卫生—药学]