微囊化胰岛B细胞系体外生长和分泌功能的研究  

STUDY OF THE GROWTH AND SECRETION OF MICROENCAPSULATED PANCREATIC B CELL LINE IN VITRO

作　　者：李崇辉[1] 罗芸[1] 李新建[1] 崔忻[1] 薛毅珑[1] 潘长玉[1] 

机构地区：[1]解放军总医院基础医学研究所,北京100853

出　　处：《中国应用生理学杂志》2001年第1期93-96,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目!(9395 70 72 5 )

摘　　要：目的 :研究海藻酸钠 多聚赖氨酸 海澡酸钠 (APA)微囊化胰岛B细胞系BTC6 F7的生长和分泌规律 ,探索其作为生物人工胰岛的可能性。方法 :以微囊静电液滴发生器制作APA微囊化BTC6 F7细胞 ,体外培养并定期测定微囊化细胞的生长和胰岛素分泌。结果 :在实验观察的 90d内 ,BTC6 F7细胞可在微囊内以细胞团的形式生长、存活。囊内细胞总数随培养时间的延长而增加 ,但细胞活率呈下降趋势 ,胰岛素分泌与囊内活细胞数的变化规律一致 ,最初呈上升趋势 ,然后较长时间维持在相对恒定的水平。结论 :本研究所制备的APA微囊化胰岛B细胞可在较长时间内保持生长、存活和分泌功能 ,为进一步发展生物型人工胰岛奠定了基础 。Aim: To study the growth and secretion of pancreatic B cell line BTC6 F7 in the alginatepolylysine alginate (APA) microcapsules and explore the possibility of utilizing it as bioartificial islets. Methods: Microencapsulated BTC6 F7 cells prepared with electric droplet generator were cultured in vitro , and the growth and insulin secretion of them were measured periodically. Results: Over the 90 day observation period, BTC6 F7 cells were able to grow and survive as cell clusters in the microcapsules and finally fulfill the capsules. But the microcapsules remained well after the long time culture. The average tota1 cell number per capsule were increasing with time, but the cell viability was decreasing. The changes of insulin secretion and average live cell number per capsule were in a same manner: for the first two weeks they increased rapidly and then maintained in a relatively constant levels for the rest time. Conclusion: The microencapsulated pancreatic B cells prepared by us could survive, grow and secretion for a long time. This has made a basis for further development of bioartiticial islets, and also can be used to study the mechanism and therapy of diabetes mellitus.

关 键 词：微囊 胰岛B细胞 糖尿病 激素缺乏症 分泌功能 

分 类 号：R587.1[医药卫生—内分泌]