丙酸高产菌株的选育及发酵培养基优化  

Screening of High Yield Propionic Acid Producing Strain and Optimization of Its Fermentation Medium

作　　者：陈唤蛟 李小连[1,2] 李彦良[1,2] 王自强[2] 王云山[2] 张利平[1] 苏志国[2] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]中国科学院过程工程研究所生化工程国客重点实验室,北京100190

出　　处：《过程工程学报》2014年第3期482-486,共5页The Chinese Journal of Process Engineering

基　　金：国家重点基础研究发展计划(973)基金资助项目(编号:2013CB733604)

摘　　要：通过环境压力筛选获得了一株耐受30 g/L丙酸的产酸丙酸杆菌菌株(Propionibacterium acidipropionici WY320),降低了发酵过程中丙酸的反馈抑制作用,采用正交实验设计优化了发酵培养基.结果表明,发酵培养基最适的玉米浆、酵母膏和(NH4)2SO4浓度分别为60,10和7.5 g/L.该条件下,摇瓶培养耐酸菌株的发酵周期为240 h,丙酸产量为49.35 g/L,较优化前提高72.98%,产率为0.21 g/(L?h);5 L发酵罐培养的发酵周期为168 h,丙酸产量达51.96g/L,产率为0.31 g/(L?h),较摇瓶培养提高47.62%,优于文献报道水平.A propionic acid-resistant strain Propionibacterium acidipropionici WY320, which could tolerate up to 30 g/L of propionic acid, was obtained by environmental stress screening to reduce the feedback inhibition of propionic acid. Its fermentation medium was optimized by orthogonal experiments to improve the propionic acid yield. The results show that the optimal concentrations of corn steep powder, yeast extract and（NH4）2SO4 were 60, 10 and 7.5 g/L, respectively. The propionic acid production by the propionic acid-resistant strain could reach 49.35 g/L, which had a significant improvement of 72.98%, with a productivity of 0.21 g/（L·h）. The scale-up experiment was carried out in a 5-L fermentor. The maximum propionic acid concentration of 51.96 g/L was obtained, and the productivity of propionic acid achieved to 0.31 g/（L·h）, increased by 47.62% compared with the result of shaking flask experiment.

关 键 词：产酸丙酸杆菌 丙酸 菌种选育 正交设计 发酵 

分 类 号：Q931[生物学—微生物学] Q815