依托咪酯和氯胺酮合用对小鼠学习记忆的影响  

Effects of etomidate combined with ketamine on learning and memory in mice

作　　者：张璐[1] 刘婷婷[1] 王丹[1] 张耀文[1] 掌曼青 吴芹[1] 浦建 滕倩[1] 章海荣[1] 马涛[2] 

机构地区：[1]徐州医学院麻醉学院2009级4班,江苏徐州221004 [2]徐州医学院麻醉药理学教研室

出　　处：《徐州医学院学报》2014年第6期371-373,共3页Acta Academiae Medicinae Xuzhou

基　　金：2012年江苏省高等学校大学生实践创新训练计划（2012JSSPZTPl835）

摘　　要：目的 观察依托咪酯（etomidate,Eto）和氯胺酮（ketamine,Ket或K）合用对小鼠学习记忆的影响.方法 实验共用昆明种小鼠80只：避暗法（step-through text）和跳台法（step-down text）各40只,再分为4组（n=10）：生理盐水（NS）组、依托咪酯（Eto）组、氯胺酮（Ket）组和两药合用（Eto＋ K）组,分别观察各组潜伏期和错误次数.结果 Eto＋K组用药24 h后避暗潜伏期（29.1±15.7）s短于NS组[（193±112）s,P＜0.01]、Eto组[（127±124）s,P＜0.05]和Ket组[（197±133）s,P＜0.01],错误次数（1.9±0.6）多于NS组（0.7±0.7,P＜0.01）、Eto组（1.1±0.9,P＜0.05）和Ket组（0.7±1.1,P＜0.01）;Eto＋K组用药48 h后避暗法潜伏期（147±134）s短于NS组[（256±78.0）s,P＜0.05]和Eto组[（252±79.1）s,P＜0.05],错误次数差异无统计学意义（P＞0.05）;Eto＋K组用药24 h后跳台法潜伏期（133±126）s短于NS组[（253±72.9）s,P＜0.01]、Eto组[（235±72.0）s,P＜0.05]和Ket组[（224±67.9）,P＜0.05],错误次数（1.3±1.1）多于NS组（0.4±0.5,P＜0.05）和Eto组（0.5±0.5,P＜0.01）;Eto ＋K组用药48 h后各组潜伏期和错误次数的差异无统计学意义（P＞0.05）.结论 依托咪酯和氯胺酮合用后至少24 h内抑制小鼠学习记忆呈协同作用,但用药后48 h协同作用减弱,不会造成持久的学习记忆障碍。Objective To observe the effects of a combination of etomidate （Eto） and ketamine （Ket or K） on learning and memory in mice.Methods 80 Kunming mice were divided into two groups for step-through and step-down tests （40 in each）.The mice in each group were subdivided into four groups （n =10）：a normal saline （NS）group,an etomidate （Eto） group,a ketamine （Ket） group and a combined treatment （Eto ＋ K） group.Then,the latency and error times of each group were observed.Results Twenty-four hours after injection,the latency in step-through test of the Eto ＋ K group （29.1 ± 15.7） s were shorter than those of the NS group [（193 ± 112） s,P 〈 0.01）],the Eto group [（127 ± 124） s,P 〈 0.05],or the Ket group [（197 ± 133） s,P 〈 0.01].Meanwhile the Eto ＋ K group presented more error times （1.9 ± 0.6） than the NS group （0.7 ± 0.7,P 〈 0.01）,the Eto group （1.1 ± 0.9,P 〈0.05）,and the Ket group （0.7 ± 1.1,P 〈0.01）.Forty-eight hours after injection,the latency in step-through test of the Eto ＋ K group （147 ± 134） s were remarkably shorter than those of the NS group [（256 ± 78.0） s,P 〈 0.05] or the Eto group [（252 ± 79.1） s,P 〈 0.05],while no statistical differences were found in error time among these groups （P 〉 0.05）.Twenty-four hours after administration,the latency in step-down test of the Eto ＋ K group （133 ± 126） s were shorter than those of the NS group [（253 ± 72.9） s,P 〈 0.01],the Eto group [（235 ± 72.0） s,P 〈 0.05],or the Ket group （224 ± 67.9）.Meanwhile,more error times were observed in the Eto ＋ K group （1.3 ± 1.1） than the NS group （0.4 ± 0.5,P 〈 0.05） and the Eto group （0.5 ± 0.5,P 〈 0.01）.No statistical differences were seen in the step-down latency and error time of the Eto ＋ K group 48 hours after administration （P 〉 0.05）.Conclusion A combination of etomidate and ketamine produces synergistic effects on learning and memory damage in mice within 24

关 键 词：依托咪酯 氯胺酮 避暗实验 跳台实验 学习记忆 

分 类 号：R614[医药卫生—麻醉学] R971[医药卫生—外科学]