谷胱甘肽在高岭土修饰碳糊电极上的电化学研究  

Electroanalysis of Glutathione at Kaolin Modified Carbon Paste Electrode

作　　者：黄娅婷[1] 黄颖[1] 潘芳芳[1] 

机构地区：[1]福建师范大学化学与化工学院,福建福州350007

出　　处：《分析测试学报》2014年第6期714-718,共5页Journal of Instrumental Analysis

基　　金：福建省属高校科研专项基金(JK2010008);福建省大学生创新创业计划(201310394066)

摘　　要：制备了高岭土修饰碳糊电极（KCPE）,并以氢醌（HQ）为电催化介质测定还原型谷胱甘肽（GSH）。实验结果表明,在该电极上谷胱甘肽（GSH）的加入对氢醌的电化学响应有明显提高,且电催化氧化峰电流与GSH浓度呈良好的线性关系,由此可采用差分脉冲伏安法（DPV）对GSH进行定量测定。对修饰电极的电化学性能进行了表征,测得电极有效表面积为0.118 6 cm2、电荷传递系数为0.68、表观速率常数为1.08×104cm3·mol-1·s-1。在优化条件下,测得GSH在KCPE上的线性范围为0.1～1,2.5～25μmol/L,检出限为0.073μmol/L。用该方法对含GSH的市售药进行测定,结果满意。Kaolin was used as a modifier for the fabrication of high electrochemical performance kaolin composite carbon paste electrode（ KCPE）,and hydroquinone（ HQ） as a new mediator was applied in indirect electrochemical determination of glutathione（ GSH）. The results showed that GSH can distinctly increase the peak current of HQ at KCPE,and the electrocatalytic current was linear with concentration of added GSH. Furthermore,the properties of modified electrode were characterized. Cyclic voltammetry（ CV）,tafel and chronoamperograms（ CA） were used to determine the effective surface area（ 0. 118 6 cm2）,charge transfer coefficient（ 0.68） and catalytic rate constant（ 1. 08 ×104cm3·mol-1·s-1）,respectively. The good linear relationship between electrocatalytic current and concentration of GSH was obtained in the ranges of 0. 1-1 μmol /L（ r2= 0. 999 5） and2. 5-25 μmol /L（ r2= 0. 999 1） with a detection limit（ S /N = 3） of 0. 073 μmol /L by differential pulse voltammetry（ DPV） under the optimal conditions. The proposed method was successfully applied in the determination of GSH in eye drops.

关 键 词：谷胱甘肽 氢醌 高岭土 碳糊修饰电极 间接电化学 

分 类 号：O657.1[理学—分析化学] O629.72[理学—化学]