美花红千层的组织培养研究  

Study on Tissue Culture of Callistemon citrinus

作　　者：舒婷[1] 

机构地区：[1]福建省林业科技试验中心,福州350000

出　　处：《安徽林业科技》2014年第2期29-33,共5页Anhui Forestry Science and Technology

摘　　要：以美花红千层茎段为外植体,进行腋芽诱导以及增殖、生根、炼苗移栽试验,以建立高效的美花红千层离体快繁体系。结果表明:以美花红千层单芽茎段在MS+6-BA0.5mg/L培养基上萌芽效果最好,芽诱导率为87%;试管苗在改良MS(NH4NO3减半)+6-BA0.3mg/L+NAA0.1mg/L培养基上继代效果最好;试管苗在1/2MS+IBA0.3mg/L+NAA0.1mg/L培养基上生根效果最好,生根率为99.3%;试管苗经炼苗,移栽至珍珠岩和腐质土3∶1混合基质中成活率最高,为95%。In this paper experiments were carried out on axillary bud induction, multiplication, rooting, acclimation and transplantation using Callistemon citrinus stem segments as explants in order to establish a rapid in-vitro propagation system of Callistemon citrinus. The results showed that the Callistemon citrinus stem segments had the best sprouting effects in the medium of MS＋6-BA0.5mg/L＋NAA0.1mg/L＋ sucrose 30g/L and the induced sprouting rate was 87%. The tube plantlets had the best subculture multiplicationeffects in the modified medium of MS＋6-BA0.3mg/L＋NAA0.1mg/L＋sucrose30g/L and the best rooting effects in the medium of 1/2MS＋IBA0.3mg/L＋NAA 0.1mg/L with the rooting rate of 99.3%. The survival rate of the tube plantlets transplanted to the mixed matrix with 3 perlite and 1 humus after acclimation was the highest and the survival rate was 95%.

关 键 词：美花红千层 腋芽诱导 继代增殖 生根诱导 炼苗移栽 

分 类 号：S685[农业科学—观赏园艺]