常见无机盐对aPTT、PT、TT测定的影响  

Effect of Inorganic Salts on the Determination of aPTT,PT and TT

作　　者：孙科[1] 孙雪[1] 王雄飞[1] 詹雪艳[1] 袁瑞娟[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《血栓与止血学》2014年第3期132-134,共3页Chinese Journal of Thrombosis and Hemostasis

基　　金：北京市自然科学基金(7133247)

摘　　要：目的探讨常用无机盐对活化部分凝血酶时间(aPTT)、凝血酶原时间(PT)和凝血酶时间(TT)的影响。方法以不同浓度的NaCl、KCl、NaHCO3、NaH2PO4为样品测定aPTT、PT和TT。结果四种无机盐能够明显延长aPTT、PT、TT时间,呈现出体外抗凝作用,作用强度与浓度呈正相关。四种盐抗凝能力强弱如下:NaH2PO4>NaHCO3>NaCl>KCl。结论无机盐会参与到凝血过程,对aPTT、PT和TT产生影响,如果样品中含有无机盐,应考虑到其影响。Objective To investigate the effect of commonly used inorganic salts on the determination of activated partial thromboplastin time（ aPTT）,prothrombin time（ PT） and thrombin time（ TT）. Methods Different concentrations of NaCl,KCl,NaHCO3,NaH2PO4were used as samples to measure aPTT,PT and TT.Results Four kinds of inorganic salt can obviously prolong the time of aPTT,PT and TT,showing significant anticoagulant effects in vitro. The anticoagulant effect was positively correlated with the concentration and the anticoagulation ability of four tested inorganic salts was as follows： NaH2PO4〉 NaHCO3〉 NaCl〉 KCl. Conclusion The inorganic salts participated in the coagulation process and affected aPTT,PT and TT,which should be taken into consideration if the samples contain inorganic salt.

关 键 词：无机盐 活化部分凝血酶时间 凝血酶原时间 凝血酶时间 

分 类 号：R446.11[医药卫生—诊断学]