响应曲面法优化泡沫分离枸杞酸性多糖  

Optimization of Foam Separation of Lycium barbarum Acidic Polysaccharides by Response Surface Methodology

作　　者：陈亮[1] 张炜[1] 陈元涛[1] 李轩领 肖江[1] 马福林[1] 

机构地区：[1]青海师范大学化学系,西宁810008

出　　处：《天然产物研究与开发》2014年第6期926-931,共6页Natural Product Research and Development

基　　金：科技部科技人员服务企业项目(2009GJG20039);青海省重点科技攻关项目(2007-N-120)

摘　　要：本文采用泡沫分离法纯化枸杞酸性多糖。在单因素实验基础上,采用Design-Expert软件中的Box-Behnken Design设计考察稀释倍数n、表观气浮速率G和浮选时间t三个因素对枸杞酸性多糖富集比和回收率的影响,建立了各因素对其综合影响的回归方程,通过该方程预测了其最佳工艺条件为:稀释倍数为10、表观气浮速率为207 mL/min,浮选时间为62 min。在此条件下枸杞酸性多糖的富集比和回收率分别为3.61%和81.76%,验证值分别为3.81%和82.47%,与预测值的相对误差分别为5.5%和0.87%。In this study, a novel method for purifying acidic polysaccharides from Lycium barbarum by foam separation was established. Based on the single factor experiment, Box-Behnken design in Design-Expert software was adopted to investigate the influence of three factors ： dilution times （n） , superficial gas floating rate （G） and floating time （t）, on enrichment ratio and recovery of acidic polysaccharides. The regression equation showed that under the optimized conditions, when dilution times （n） was 10, superficial gas floating rate （G） was 207 mL/min, floating time （t） was 62 rain, enrichment ratio and recovery of acidic polysaccharides reached highest to 3.61 and 81.76%. The relative error between verification values （ 3.81% and 82.47% ） and theoretic values were 5.5% and 0.87% , respectively.

关 键 词：枸杞多糖 糖醛酸 泡沫分离 响应曲面法 

分 类 号：X703[环境科学与工程—环境工程]