新疆妇女宫颈癌前病变中高危HPV型别与HPVL1壳蛋白表达的相关性研究  

Correlation of high risk HPV type and HPV L1 capsid protein expression in cervical lesions in Xinjiang

作　　者：梁媛哲 玛依努尔.尼亚孜 朱开春[2] 

机构地区：[1]石河子大学医学院,新疆石河子832000 [2]新疆维吾尔自治区人民医院,新疆乌鲁木齐830000

出　　处：《现代预防医学》2014年第13期2359-2361,共3页Modern Preventive Medicine

基　　金：国家自然科学基金资助项目(新疆维吾尔族妇女子宫颈癌早期预警因子的研究No:81160317)

摘　　要：目的了解汉族、维吾尔族妇女宫颈癌前病变中不同HPV亚型L1蛋白的表达差异。方法收集HPV阳性的宫颈脱落细胞标本共248例,其中汉族、维吾尔族慢性宫颈炎各48例、46例,CINI各40例、30例,CINII-III各28例、56例。提取HPV DNA进行PCR扩增,采用导流杂交基因芯片技术检测HPV基因型,石蜡切片免疫组化法检测HPV L1蛋白。结果248例患者共检测出15种HPV亚型,随着病变程度的加重,HPV亚型趋于集中,HPV16型检出率最高;维吾尔族HPV16、非HPV16高危亚型中HPV L1蛋白的表达率分别为23.26%(20/86)、43.48%(20/46),差异有统计学意义(P=0.016),HPV16型中汉族与维吾尔族HPV L1蛋白的表达率分别为42.5%(34/80)、23.26%(20/86),差异亦有统计学意义(P=0.008)。结论维吾尔族HPV16型L1蛋白的表达明显低于非HPV16高危亚型,提示HPV L1蛋白的表达缺失可能为宫颈病变中HPV16型感染率最高的原因之一;HPV16型中维吾尔族HPV L1蛋白的表达显著低于汉族,提示HPV L1蛋白的表达缺失可能为维吾尔族宫颈病变患病率高于汉族的原因之一。Objective To explore the expression of different HPV isoforms of L1 protein in the cervical lesions of Han, Uygur women.Methods Collection of HPV positive cervical exfoliated cell specimens of 248 cases, including 48 cases, 46 cases of chronic cervicitis, 40 cases, 30 cases of CINI, 28 cases, 56 cases of CINII-III in Han and Uygur respectively. DNA was extracted for PCR amplification, HPV genotypes were detected using flow-through hybridization gene chip technology, determination of HPV L1 protein from paraffin used immunohistochemical staining. Results 248 cases were detected in 15 kinds of HPV subtypes. As the severity of lesions, HPV types tended to be focused. The type of HPV16 had the highest detection rate; the expression rate of HPV L1protein in HPV16, non- HPV16 high risk subtypes of the Uygur was 23.26%（20/86）, 43.48%（20/46）, the difference was significant（P=0.016）, the expression rate of HPV L1 protein in HPV16 of the Han was 42.5%（34/80）, 23.26%（20/86）, the difference was significant（P=0.008）. Conclusion Expression of HPV16 L1 protein is significantly lower than the non-HPV16 high-risk subtypes of Uygur,. The absence of expression of HPV L1 protein in cervical lesions may be one of the reasons for HPV16 highest infection rate;expression of L1 protein in HPV16 is significantly lower in the Uygur than the Han. The absence of expression of HPV L1 protein may be one of the reasons for the Uigur cervical disease prevalence is higher than the Han nationality.

关 键 词：人乳头瘤状病毒 L1壳蛋白 宫颈病变 

分 类 号：R17[医药卫生—妇幼卫生保健]