HPV16E7,E5序列多态性与新疆地区维汉妇女宫颈癌及癌前病变的相关性研究  

Study on correlation of HPV16E7, E5 sequence polymorphism and the Xinjiang Uygur and Han cervical cancer and precancerous lesions

作　　者：杨丹[1] 玛依努尔.尼亚孜 朱开春[3] 赵宗峰[3] 

机构地区：[1]新疆石河子大学医学院,新疆石河子832000 [2]新疆维吾尔自治区人民医院妇科,新疆乌鲁木齐830001 [3]新疆维吾尔自治区人民医院妇产科研究室,新疆乌鲁木齐830001

出　　处：《现代预防医学》2014年第13期2365-2368,共4页Modern Preventive Medicine

基　　金：国家自然科学基金项目(81160317)

摘　　要：目的分析新疆地区宫颈病变组织中HPV16E7,E5基因序列变异及该变异与宫颈病变的相关性,探讨该变异率在新疆地区维,汉妇女不同宫颈病变中的差异。方法对HPV16E7,E5DNA阳性的119例宫颈炎、70例CINI、91例CINII-CINIII和105例宫颈癌的基因片段进行PCR扩增测序,分析突变情况。结果除了HPV16E7,E5的原型序列以外,HPV16E7最常见的变异为A647G和T846C,主要以A647G突变为主,A647G变异株频率在宫颈炎、CINI、CINII-CINIII和宫颈癌分别为维22.2%、60.0%、57.8%、55.3%;汉24.6%、55.0%、60.9%、66.7%。HPV16E5最常见的变异为A4042G和A3978C,主要以A4042G突变为主,A4042G变异株频率在宫颈炎、CINI、CINII-CINIII和宫颈癌分别为维18.5%、53.3%、53.3%、55.0%;汉21.5%、45.0%、56.5%、68.9%。HPV16E7A647G变异株和HPV16E5A4042G变异株在新疆地区维,汉妇女各个宫颈病变阶段中的分布差异有统计学意义(P<0.05),比较该变异株在维,汉妇女宫颈病变各个阶段中的分布差异无统计学意义(P>0.05)。结论HPV16E7A647G变异株和HPV16E5A4042G变异株是新疆地区宫颈病变中流行的变异株,此变异株与宫颈病变的程度有相关性,在新疆地区宫颈病变中无民族差异性。Objective To analyze the correlation of HPV16E7, E5 gene sequence variation and cervical lesions in Xinjiang region,to investigate the differences in mutation rate in the Xinjiang Uygur, Han women with different cervical lesions. Methods The gene fragments from positive HPV16E7 and E5DNA of 119 cases of cervicitis, 70 cases of CINI, 91 cases CINII-CIN III and 105 cases of cervical cancer were amplified by PCR sequencing, and analyzed mutation. Results In addition to the prototype sequence of HPV16E7, E5, the most common variants of HPV16E7 were A647G and T846C, main mutation in A647G. The A647G mutant frequency in cervicitis, CINI, CINII-CINIII and cervical cancer were 22.2% and 60.0%, 57.8%, 55.3% in Uighurs; 24.6%, 55.0%,60.9%, 66.7% in Han. Most common mutations of HPV16E5 were A4042G and A3978C, based mainly mutation was A4042G.A4042G mutant frequency in cervicitis, CINI, CINII-CINIII and cervical cancer were 18.5%, 53.3%, 53.3%, 55.0% in Uighurs;21.5%, 45.0%, 56.5%, 68.9% in Han. The distribution difference of HPV16E7A647G and HPV16E5A4042G mutants in various stages of cervical lesions of women in Uygur, Han in Xinjiang was statistically significant（P〈0.05）. Comparing the difference in the distribution of variants in various stages of Uygur and Han women with cervical lesions, there was no statistical significance（P〉0.05）. Conclusion HPV16E7A647G and HPV16E5A4042G are popular variants in cervical lesions in Xinjiang region, mutants are correlated with the degrees of cervical lesions, without racial difference in cervical lesions in Xinjiang region.

关 键 词：HPV16E7和E5 序列多态性 宫颈癌 

分 类 号：R17[医药卫生—妇幼卫生保健]