海南省发热病人标本中斑点热立克次体groEL基因检测  

Detection of groEL gene in spotted fever group rickettsia of patients with fever in Hainan province

作　　者：吴捷[1,2] 王少玲[2] 金玉明[2] 马焱[2] 曾祥洁[2] 贾鹏本[2] 吴维学[2] 苏新元[2] 李沅[2] 聂绍发[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院,湖北武汉430030 [2]海南省疾病预防控制中心,海南海口570203

出　　处：《现代预防医学》2014年第13期2417-2419,共3页Modern Preventive Medicine

基　　金：海南省自然科学基金(808225);海南省卫生厅科学研究课题(琼卫2008-72)

摘　　要：目的了解海南省发热病人中感染斑点热群立克次体的状况。方法利用巢氏PCR方法,检测海南省2009-2010年医院送检的117份不明原因发热病人血标本的groEL基因的特异性片段,对所检测到的部分阳性结果进行序列测定,并进行聚类分析,分析海南省斑点热群立克次体优势株的流行情况。结果从117份发热病人血标本中发现有18份groEL阳性,取12份groEL阳性核酸产物进行测序,7份核酸序列基本一致。结论海南省发热病人中持续存在斑点热群立克次体感染的状况,应加强对斑点热的实验室检测能力,为其防治提供有力依据。Objective To understand the station of spotted fever group rickettsia infection of patients with fever in Hainan province.Methods Specific fragment of blood samples of groEL gene was detected by the nested PCR method; the samples were collected from 117 patients with unknown fever in hospitals of Hainan province during 2009-2010. Parts of the positive samples were sequenced and clustered, the epidemic situation of dominant strains of potted fever group rickettsia was analyzed in Hainan province.Results 18 groEL positive samples were found from 117 samples of blood, 12 samples were sequenced, in which 7 with the same sequences. Conclusion Potted fever group rickettsia exists in patients with fever in Hainan, it should strengthen the ability of detecting spotted fever in the lab, so as to provide basis for its prevention and control.

关 键 词：斑点热群立克次体 热休克蛋白groEL基因 聚合酶链反应 

分 类 号：R181.33[医药卫生—流行病学]