吡啶羧酸铬对生长期笼养蛋鸭免疫器官指数和抗氧化功能的影响  

Effects of dietary chromium picolinate level on immune organ index and antioxidant function of growing laying ducks

作　　者：田世勋[1] 袁艺森[1] 李越[1] 谭玲芳[1] 王安[1] 

机构地区：[1]东北农业大学动物营养研究所,黑龙江哈尔滨150030

出　　处：《中国饲料》2014年第12期34-37,40,共5页China Feed

基　　金：国家自然科学基金委资助(30972111)

摘　　要：为研究不同水平的吡啶羧酸铬对生长期（5-10周龄）笼养蛋鸭免疫器官指数和抗氧化功能的影响,试验采用单因素完全随机分组设计,选择28日龄健康金定蛋鸭180只,随机分成5组,每组6个重复,每个重复6只鸭。对照组（Ⅰ组）饲喂玉米-豆粕型基础日粮,试验组（Ⅱ-Ⅴ组）分别在基础日粮中添加0.15、0.30、0.45、0.60 mg/kg铬（吡啶羧酸铬）。试验期6周。结果表明：（1）Ⅱ、Ⅲ、Ⅳ组的胸腺指数分别比Ⅰ组提高27.65%、29.33%、24.30%（P〈0.05）,但对脾脏指数和法氏囊指数未产生显著影响（P〉0.05）。（2）添加适量的吡啶羧酸铬可提高肝脏的总抗氧化能力,Ⅲ组最高,分别较Ⅰ、Ⅴ组提高12.04%、10.44%（P〈0.05）,Ⅳ组血清中总超氧化物歧化酶的活性比Ⅰ组提高4.70%（P〈0.05）,Ⅲ组肝脏中总超氧化物歧化酶的活性较Ⅰ、Ⅴ组分别提高4.93%、3.98%（P〈0.05）,Ⅱ、Ⅲ、Ⅳ组血清中丙二醛的含量分别比Ⅰ组降低9.35%、10.79%、10.07%（P〈0.05）。但添加吡啶羧酸铬对谷胱甘肽过氧化物酶的活性影响不显著（P〉0.05）。通过二次回归曲线分析表明铬（以吡啶羧酸铬形式添加）在生长期笼养蛋鸭日粮中最适宜的添加量为0.3189-0.3492 mg/kg。The experiment was conducted to study effects of different levels of chromium as chromium picolinate on immune organ index and antioxidant function of caged growing laying ducks aged from 5 to 10 weeks.Using a single factor completely randomized design,a total of 180 28-day-old Jin-ding laying ducks were randomly allocated to 5 groups with 6 replicates in each group.The control group （group Ⅰ ）was fed corn-soybean meal basal diet,and the experimental groups （groups Ⅱ - V ）were fed corn-soybean meal basal diet supplemented with 0.15,0.30,0.g5 mg/kg and 0.60 mg/kg chromi- um as chromium picolinate respectively.The experimental period lasted for 6 weeks.The results showed as follows ： （ 1 ）Thymus index of group Ⅱ , Ⅲ,Ⅳ increased by 27.65%,29.33% and 24.30% compared with group I respectively（P 〈 0.05）, but there was no significant effect on spleen index and bursa index （P 〉 0.05）.（2）The total anti-oxidant capacity （T- AOC） in liver of group Ⅲ was the highest and increased by 12.04% and 10.44% compared with group Ⅰ , V respectively （P 〈 0.05）,and the activity of total super oxide dismutase （T-SOD） in serum of group IV increased by 4.70% compared with group Ⅰ （P 〈 0.05） and the activity of total superoxide dismutase （T-SOD）in liver of group Ⅲ increased by 4.93% and 3.98% compared with group Ⅰ , V respectively （P 〈 0.05）.The contents of serum malondialdehyd（MDA）of group Ⅰ , 11I, IV reduced by 9.35%, 10.79% and 10.07% compared with group I respectively （P 〈 0.05）.But the activity of glutathion peroxidase（GSH-Px）was insignificantly different from each other（P 〉 0.05）.The results indicated that the optimum adding level of chromium as chromium picolinate in the diet of caged growing laying ducks was 0.3189 - 0.3492 mg/kg by fitting a quadratic regression curve.

关 键 词：吡啶羧酸铬 生长期蛋鸭 免疫器官指数 抗氧化酶 

分 类 号：S816.7[农业科学—饲料科学]