AT1R和eNOS在盐敏感性高血压发生中作用  

Effect of AT1R and eNOS on salt-sensitive hypertension in rats

作　　者：张娟[1] 白宝琴[2] 李莉 殷丽天[1] 关丽[1] 杨建一[1] 

机构地区：[1]山西医科大学基础医学院细胞生物与遗传学教研室,山西太原030001 [2]大同市第三人民医院

出　　处：《中国公共卫生》2014年第7期912-915,共4页Chinese Journal of Public Health

基　　金：山西省自然科学基金(200811076-1)

摘　　要：目的探讨血管紧张素Ⅱ1型受体(AT1R)和内皮型一氧化氮合酶(eNOS)在盐敏感性高血压发生发展中的作用。方法用乳鼠皮下注射辣椒辣素法建立模型。哺乳期后,大鼠随机分成4组:对照组、高盐组、辣椒辣素组、辣椒辣素+高盐组,喂养4周后处死大鼠,免疫组化检测大鼠腹主动脉AT1R和eNOS蛋白表达,反转录-聚合酶链式反应检测大鼠腹主动脉AT1R和eNOS mRNA表达。结果与对照组比较,辣椒辣素组大鼠动脉AT1R蛋白表达量增加0.70倍(P<0.01),而动脉eNOS蛋白表达量降低20.5%(P<0.05),eNOS mRNA的表达降低0.80倍(P<0.05);与对照组比较,辣椒辣素+高盐组大鼠动脉AT1R蛋白表达量增加1.69倍(P<0.01),AT1R mRNA表达量提高1.70倍(P<0.01),而动脉eNOS蛋白表达量降低60.2%(P<0.01),eNOS mRNA表达降低2.52倍(P<0.01)。结论感觉神经损伤性盐敏感性高血压大鼠动脉AT1R表达升高,同时eNOS表达降低,AT1R和eNOS表达水平的差异可能与盐敏感性高血压的形成有关。Objective To explore the role of angiotensin [[ 1 receptor（ AT1 R）and endothelial nitric oxide synthase （eNOS） in the development of salt-sensitive hypertension. Methods Newborn male Wistar rats were given capsaicin subcutaneously to establish the model. After weaning period, the rats were divided into 4 groups and fed with different salt diets for 4 weeks-control of normal sodium diet（ CON-NS）, control of high sodium diet（ CON-HS ）, capsaicin pretreatment plus normal sodium diet （ CAP-NS ）, and capsaicin pretreatment plus high sodium diet （ CAP-HS ）. The rats were killed after 4 weeks＇ treatment. Immunohistochemistry was performed to detect the expression of AT1R and eNOS proteins in the artery. The expression of AT1R and eNOS mRNA in the artery was determined by reverse transcription polymerase chain reaction. Results Compared with the CON-NS rats,the expression of AT1R protein in the artery was remarkably higher in the CAP-NS rats （P 〈 0. 01 ）, while the expression of eNOS mRNA and protein in the artery were remarkably decreased in the CAP-NS rats （ P 〈 0. 05 ）. Compared with the CON-NS rats, the expression of AT1R mRNA and protein in the artery was remarkably higher in the CAP-HS rats （ P 〈 0. 01 ）, while the expression of eNOS mRNA and protein in the artery was remarkably decreased in the CAP-HS rats （ P 〈 0. 01 ）. Conclusion The expression of AT1R mRNA and protein increase, and the expression of eNOS mRNA and protein decrease in the artery of the salt-sensitive hypertensive rats induced by sensory denervation. The different expressions of AT1R and eNOS may be related with the pathogenesis of salt-sensitive hypertension.

关 键 词：盐敏感性高血压 血管紧张素Ⅱ 1型受体(AT1R) 内皮型一氧化氮合酶(eNOS) 大鼠 

分 类 号：R33[医药卫生—人体生理学]