乌江网箱养殖加州鲈鱼致病病原的分离与鉴定  

Isolation and Identification of Pathogenic Bacteria Isolated from Micropterus salmonides Cultured in Net Cage in Wujiang River

作　　者：魏志琴[1] 杨秀荣[1] 曾燕玲[1] 宋培勇[1] 

机构地区：[1]遵义师范学院生命科学学院,贵州遵义563002

出　　处：《贵州农业科学》2014年第5期150-153,共4页Guizhou Agricultural Sciences

基　　金：贵州省遵义市科技局项目"乌江网箱养鱼细菌性疾病的中草药防治"[遵市科合社字(2007)21]

摘　　要：为探明乌江网箱养殖加州鲈鱼的致病病原,采用营养琼脂培养基分离纯化,用传统分类和16S rRNA基因序列分析方法,对其进行了分离与鉴定。结果表明：共分离出4个菌株（Ⅰ～Ⅳ）,各菌株均扩增出1条约1 500bp片段,与目的片段大小一致;菌株Ⅰ和菌株Ⅱ为溶酪巨球菌（Macrococcus caseolyticus）,菌株Ⅲ为鲁氏不动杆菌（Acinetobacter lwoffii）,菌株Ⅳ为西伯利亚微小杆菌（Exiguobacterium sibiricum）。经回接感染试验,4株细菌均能引起加州鲈鱼患病。In order to study the pathogen of infectious M.salmonides cultured in net cage in Wuj iang River,the authors conducted an identification to the suspicious pathogenic bacteria isolated by integrated method of the traditional classification of microorganisms and 16S rRNA gene comparison analysis. Results：Four strains were isolated with a 1500 bp fragment,which was of the same size with target fragment. Isolates I and II, III and IV were Macrococcus caseolyticus,Acinetobacter lwoffii and Exiguobacterium sibiricum,respectively.It proved that all the four isolates were capable of causing the fish （Micropterus salmonides）disease by reinoculation test.

关 键 词：加州鲈鱼 网箱养殖 可疑病病原菌 分类鉴定 回接试验 乌江 

分 类 号：S941.42[农业科学—水产养殖]