胡桃醌对宫颈癌caski细胞的细胞毒作用的研究  

The cytotoxicity of Juglone on caski cells

作　　者：吴亚猛 张琳娜[1] 陈思瑶[1] 韩景春[1] 那文婷[1] 张巍[2] 

机构地区：[1]吉林医药学院临床医学院,吉林吉林132013 [2]吉林医药学院生化教研室,吉林吉林132013

出　　处：《吉林医药学院学报》2014年第3期176-178,共3页Journal of Jilin Medical University

基　　金：吉林省科技厅科技发展项目(20130101157JC);吉林省教育厅"十二五"教育技术研究项目(2014-371);吉林省教育厅"十二五"教育技术研究项目(2011-286)

摘　　要：目的探讨胡桃醌对宫颈癌caski细胞增殖的影响。方法将宫颈癌caski细胞传代培养至对数生长期,分组加入不同浓度的胡桃醌,并设空白对照组,用MTT比色法观察胡桃醌对体外培养的宫颈癌caski细胞生长的抑制作用,光学显微镜观察细胞形态学变化。结果 MTT比色法测定显示,胡桃醌作用于宫颈癌caski细胞后可以抑制其增殖且呈剂量依赖性。结论胡桃醌能抑制体外培养的宫颈癌caski细胞的增殖。Objective To explore the inhibitory effect of the Juglone on the proliferation of cervical cancer caski cells. Methods Caski cells were cultured to the logarithmic growth period and were treated with different concentra- tion of Juglone. The inhibitory effect of Juglone on cells was measured by MTT assay and the morphological changes of cells were also observed under optical microscope. Results MTT assay showed that Juglone could inhibit the proliferation of caski cells in a dose-dependent manner. Conclusion Juglone can inhibit the proliferation of caski cells in vivo.

关 键 词：胡桃醌 CASKI细胞 增殖 

分 类 号：R285.5[医药卫生—中药学] R737.33[医药卫生—中医学]