阻断胸腺基质淋巴细胞生成素抑制Th2反应的体外研究  

Inhibition of Th2 reaction by thymic stromal lymphopoietin blockade in vitro

作　　者：温立婷[1] 李旭[2] 乔莉[1] 米文娟[1] 王烨[1] 石照辉[1] 邱建华[1] 

机构地区：[1]第四军医大学西京医院耳鼻咽喉头颈外科,西安710032 [2]中国人民解放军第二六一医院耳鼻咽喉头颈外科

出　　处：《中华耳鼻咽喉头颈外科杂志》2014年第6期488-492,共5页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：国家自然科学基金面上项目（81071791）;国家自然科学基金青年科学基金（81300808）

摘　　要：目的 观察阻断胸腺基质淋巴细胞生成素（thymic stromal lymphopoietin,TSLP）对体外培养的树突状细胞（dendritic cell,DC）及T淋巴细胞向Th2方向转化的影响.方法 小鼠未成熟DC及T淋巴细胞体外共培养,将其以随机数字表法分为4组：TSLP刺激组、TSLP刺激并TSLP受体阻断组、卵清白蛋白刺激组、卵清白蛋白刺激并TSLP受体阻断组.应用酶联免疫吸附试验（ELISA）法于共培养2d后分别检测各组细胞培养上清中白细胞介素4（IL-4、IL-8及β干扰素（IFN-β）水平.以SPSS 13.O软件进行统计学分析.结果 ELISA结果显示,TSLP刺激并TSLP受体阻断组和卵清白蛋白刺激并TSLP受体阻断组的IL-4水平分别为（48.84±1.56）、（52.53±2.36） pg/ml,明显低于TSLP刺激组及卵清白蛋白刺激组的（72.55 ±7.76）、（80.47 ±21.93） pg/ml,差异有统计学意义（t=5.994,P ＜0.05;t =2.534,P ＜0.05）.而TSLP受体阻断对细胞培养上清中IL-8及IFN-β表达水平无显著影响（P值均＞0.05）.结论 体外行TSLP受体阻断可抑制T淋巴细胞向Th2方向转化,这可能为临床变应性鼻炎及哮喘等Th2优势的疾病提供新的治疗策略.Objective To explore the effect of thymic stromal lymphopoietin （TSLP） on transformation of dendritic cell （DC） and T cell in vitro.Methods Mouse-derived immature dendritic cells and T lymphocytes were co-cultured in vitro,which were divided into 4 groups （TSLP stimulation group,TSLP stimulation and its receptor blocking group,ovalbumin stimulation group and ovalbumin stimulation and TSLP receptor blocking group）.IL-4,IL-8 and IFN-β in cell culture supernatant were detected after 2 days by ELISA.SPSS 13.0 software was used to analyze the data.Results IL-4 levels of TSLP receptor blocking groups [（48.84 ± 1.56） pg/ml,（52.53 ± 2.36） pg/ml] were significantly lower than those of corresponding TSLP stimulation group and ovalbumin stimulation group [（72.55 ± 7.76） pg/ml,（80.47 ± 21.93） pg/ml ; t =5.994,P ＜ 0.05 ; t =2.534,P ＜ 0.05].However,there were not significant differences of IL-8 and IFN-β expression between corresponding two groups of whether or not TSLP receptor blocking （all P ＞ 0.05）.Conclusion TSLP receptor blockade in vitro can inhibit T lymphocyte transformation to Th2,which may provide a new therapeutic strategy for clinical Th2 dominant diseases such as allergic rhinitis and asthma.

关 键 词：细胞因子类 树突细胞 T淋巴细胞 抗体 封闭 TH2细胞 免疫球蛋白类 受体 细胞因子 细胞 培养的 

分 类 号：R392.1[医药卫生—免疫学]