IL-lβ和TNF-α对人牙周膜细胞内LIF表达的影响  

Effects of IL-l β and TNF-α on the expression of LIF mRNA in periodontal ligament cells

作　　者：梁又德 周毅[2] 杨旭 王贻宁[2] 

机构地区：[1]深圳市第六人民医院口腔科,广东深圳518052 [2]武汉大学口腔医学院修复科,湖北武汉430079

出　　处：《临床口腔医学杂志》2014年第6期334-336,共3页Journal of Clinical Stomatology

基　　金：深圳市南山区科技基金资助(2012012)

摘　　要：目的:探讨白细胞介素-lβ(IL-lβ)和肿瘤坏死因子-α(TNF-α)对牙周膜细胞内白血病抑制因子(LIF)表达的影响。方法:体外分离培养鉴定人牙周韧带细胞(HPDLC),取第3代细胞用于实验,利用牙周改建中高表达因子TNF-α和IL-1β刺激HPDLC后,实时定量反转录-聚合酶链反应检测LIF mRNA的表达。结果:IL-1β在浓度为0.1ng/mL和5 ng/mL时,均显著促进LIF的分泌(P<0.01)。TNF-α在浓度为10 ng/mL时,明显促进LIF的分泌(P<0.05)。结论:牙周改建中高表达细胞因子IL-1β和TNF-α可促进牙周膜细胞中LIF的表达。Objective：To investigate the effects of interleukin-1β（IL-1β） and tumor necrosis factor-α（TNF-α） on the expression of leukemia inhibitory factor （LIF）mRNA in the human periodontal ligament cells （HPDLCs）. Method：Human periodntal tissue was obtained from the extracted healthy premolar for orthodontic reasons. The HPDLCs used in this study underwent three passages. The expression of LIF mRNA in HPDLCs with or wihout IL-1β and TNF-α. Treatment was determined by realtime RT-PCR. Result：The expression of LIF mRNA was significantly induced by IL-1βin both 0.1 ng/mL and 5 ng/mL （P 〈0.01）. The expression of LIF mRNA was markedly enhanced by TNF-α（P 〈0.05）. Conclusion：Cytokines such as TNF-α and IL-1β highly expressed during periodontium remolding which dramatically stimulated the secretion of LIF in HPDLCs.

关 键 词：白介素1 肿瘤坏死因子α 白血病抑制因子 牙周韧带细胞 

分 类 号：Q786[生物学—分子生物学]